Characterization of incubation experiments and development of an enrichment culture capable of ammonium oxidation under iron-reducing conditions

Huang, S.; Jaffé, P. R.

doi:https://doi.org/10.5194/bg-12-769-2015

Articles | Volume 12, issue 3

https://doi.org/10.5194/bg-12-769-2015

© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

https://doi.org/10.5194/bg-12-769-2015

© Author(s) 2015. This work is distributed under
the Creative Commons Attribution 3.0 License.

Articles | Volume 12, issue 3

Research article

|

10 Feb 2015

Research article |

| 10 Feb 2015

Characterization of incubation experiments and development of an enrichment culture capable of ammonium oxidation under iron-reducing conditions

S. Huang and P. R. Jaffé

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Final revised paper (published on 10 Feb 2015)
Supplement to the final revised paper
Preprint (discussion started on 14 Aug 2014)
Supplement to the preprint

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

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- Supplement

RC C5163: 'bg-2014-372', Anonymous Referee #1, 15 Sep 2014
- AC C5238: 'Responses to Anonymous Referee #1', Peter Jaffe, 20 Sep 2014
RC C5414: 'bg-2014-372', Anonymous Referee #2, 24 Sep 2014
- AC C5523: 'Responses to Anonymous Referee #2', Peter Jaffe, 29 Sep 2014

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

AR by Peter Jaffe on behalf of the Authors (11 Nov 2014) Author's response Manuscript

ED: Referee Nomination & Report Request started (14 Nov 2014) by Jack Middelburg

RR by Anonymous Referee #1 (19 Nov 2014)

ED: Publish subject to minor revisions (Editor review) (25 Nov 2014) by Jack Middelburg

Dear Dr. Jaffe:

Your paper has been evaluated again by one of the referees and she/he recommended publication pending minor revision. I agree based on my reading of your revised version and your rebuttal. Therefore, I am happy to accept your paper after minor corrections have been made.

The contacted referee identified quite some corrections and identified the need for English correction of the newly added sections. I fully agree and have made a list of corrections as well that you can find below. Please amend your manuscript with my and the referees’ corrections, check references, and then upload a new version that will be processed for publication: i.e. I will check and then accept. Most important is the correction of Equation 1.

Thank you for submitting this paper to Biogeosciences,

Jack Middelburg
Editor of Biogeosciences

List of corrections:

L 29-35: the flow of this newly added section is suboptimal. Please rewrite

L. 31: sediments, such as those found in wetlands and other aquatic systems, there is…. (benthic sediments make no sense).

l. 33: the link with groundwater discharging is unclear

l. 41, equation 1. The stoichiometry of your reactions is not right: you produce 7.5 water molecules not 8.5. (just check the number of O and H atoms on both sides). This is not just a typo it comes back a couple of times and most importantly your Gibbs Free energy calculations are based on this wrong stoichiometry.

L. 74: no manganese oxides

L. 118: final

L. 127: through

L. 134: fed

L. 135: dissolved

L. 140: introduce DI abbreviation upon first use

L. 185: Table 1, not Table1 (all through)

L. 217: Rotthauwe et al. 1997. Where does it refer to. This is not clear at the end of the sentence.

L. 219: 2008).

L. 244: found in the sterilized

L. 255: Fig. S6 ( is correct, but is S5 in appendix).

L. 326-331: the number of nirS gene and nirK gene. This does not make sense. Rewrite this section to the number of copies of nirS gene…
Similarly, Growth of nirS and nirK gene copies showed….

L. 368: in samples

L. 386: Fe (III) was added

L. 387: do not require, no…

L. 423 a strong link with an Feammox…

L. 445: delete might

L. 454-455: unclear. Do you mean no production?

Check reference list for completeness.

Supplementary information

Section 1.1. Equation 1 was wrong and the delG calculation as well.
L 25-28: should all be 7.5 instead of 8.5

L. 121: After 270 days of operatioin Feammox enrichment slurries were collected from the …..

L. 127: was added

L. 134: Ding et al. is not in reference list.

Line 202: Fig. 6S not 5S, also Stoichiometry…

Line 227: supplementary references: check.

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AR by Peter Jaffe on behalf of the Authors (28 Nov 2014) Author's response

ED: Publish as is (01 Dec 2014) by Jack Middelburg

AR by Peter Jaffe on behalf of the Authors (02 Dec 2014)

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Short summary

Significant changes in the microbial community were observed during 180-day incubations of soil samples from a wetland where Feammox activity has been observed previously, as well as in a continuous flow membrane reactor. Results indicate that the dominant microbial species, an uncultured Acidimicrobiaceae bacterium A6 belonging to the Acidimicrobiaceae family, plays a key role in this anaerobic biological process using ferric iron as an electron acceptor while oxidizing ammonium to nitrite.