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Articles | Volume 15, issue 12
Articles | Volume 15, issue 12
https://doi.org/10.5194/bg-15-3873-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-15-3873-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 15, issue 12
Research article
 | 
27 Jun 2018
Research article |  | 27 Jun 2018

Estimation of isotope variation of N2O during denitrification by Pseudomonas aureofaciens and Pseudomonas chlororaphis: implications for N2O source apportionment

Joshua A. Haslun, Nathaniel E. Ostrom, Eric L. Hegg, and Peggy H. Ostrom

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Short summary
N2O δ15N and δ18O values changed non-linearly during in vitro N2O production by bacterial denitrification of NO3−, an argument against their use in N2O apportionment. We present a novel approach for describing non-linear isotopic behaviour, which may be applied to other multi-step reactions. We also show that the intramolecular distribution of 15N in N2O, used to apportion N2O emissions to denitrification and nitrification, is unaffected by electron donor source and electron donor concentration.
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