Ozone (O₃) pollution reduces wheat yields and quality in India, affecting amino acids essential for nutrition, like lysine and methionine. Here, we improve the DO₃SE-CropN model to simulate wheat’s protective processes against O₃ and their impact on protein and amino acid concentrations. While the model captures O₃-induced yield losses, it underestimates amino acid reductions. Further research is needed to refine the model, enabling future risk assessments of O₃'s impact on yields and nutrition.