Biogeosciences
Biogeosciences
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Articles | Volume 22, issue 7
Articles | Volume 22, issue 7
https://doi.org/10.5194/bg-22-1729-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-22-1729-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 22, issue 7
Technical note
 | 
07 Apr 2025
Technical note |  | 07 Apr 2025

Technical note: Flow cytometry assays for the detection, counting and cell sorting of polyphosphate-accumulating bacteria

Clémentin Bouquet, Hermine Billard, Cécile C. Bidaud, Jonathan Colombet, Young-Tae Chang, Joan Artigas, Isabelle Batisson, Karim Benzerara, Fériel Skouri-Panet, Elodie Duprat, and Anne-Catherine Lehours

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Short summary
In the context of the ecological sustainability of phosphorus, the ubiquitous presence of polyphosphate-accumulating bacteria in natural environments invites efforts to reveal their unknown roles in the biogeochemical cycle of phosphorus. In this study, we evaluated the potential of combining the staining of intracellular polyphosphate granules and their subsequent detection by flow cytometry for the detection, quantification and cell sorting of polyphosphate-accumulating bacteria.
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