Technical note: Flow cytometry assays for the detection, counting and  cell sorting of polyphosphate-accumulating bacteria

Bouquet, Clémentin; Billard, Hermine; Bidaud, Cécile C.; Colombet, Jonathan; Chang, Young-Tae; Artigas, Joan; Batisson, Isabelle; Benzerara, Karim; Skouri-Panet, Fériel; Duprat, Elodie; Lehours, Anne-Catherine

doi:10.5194/bg-22-1729-2025

Articles | Volume 22, issue 7

https://doi.org/10.5194/bg-22-1729-2025

© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/bg-22-1729-2025

© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 22, issue 7

Technical note

|

07 Apr 2025

Technical note |

| 07 Apr 2025

Technical note: Flow cytometry assays for the detection, counting and cell sorting of polyphosphate-accumulating bacteria

Clémentin Bouquet, Hermine Billard, Cécile C. Bidaud, Jonathan Colombet, Young-Tae Chang, Joan Artigas, Isabelle Batisson, Karim Benzerara, Fériel Skouri-Panet, Elodie Duprat, and Anne-Catherine Lehours

Related authors

Biomineralization of amorphous Fe-, Mn- and Si-rich mineral phases by cyanobacteria under oxic and alkaline conditions

Karim Benzerara, Agnès Elmaleh, Maria Ciobanu, Alexis De Wever, Paola Bertolino, Miguel Iniesto, Didier Jézéquel, Purificación López-García, Nicolas Menguy, Elodie Muller, Fériel Skouri-Panet, Sufal Swaraj, Rosaluz Tavera, Christophe Thomazo, and David Moreira

Biogeosciences, 20, 4183–4195, https://doi.org/10.5194/bg-20-4183-2023,https://doi.org/10.5194/bg-20-4183-2023, 2023

Short summary

The hidden role of dissolved organic carbon in the biogeochemical cycle of carbon in modern redox-stratified lakes

Robin Havas, Christophe Thomazo, Miguel Iniesto, Didier Jézéquel, David Moreira, Rosaluz Tavera, Jeanne Caumartin, Elodie Muller, Purificación López-García, and Karim Benzerara

Biogeosciences, 20, 2405–2424, https://doi.org/10.5194/bg-20-2405-2023,https://doi.org/10.5194/bg-20-2405-2023, 2023

Short summary

Biogeochemical processes captured by carbon isotopes in redox-stratified water columns: a comparative study of four modern stratified lakes along an alkalinity gradient

Robin Havas, Christophe Thomazo, Miguel Iniesto, Didier Jézéquel, David Moreira, Rosaluz Tavera, Jeanne Caumartin, Elodie Muller, Purificación López-García, and Karim Benzerara

Biogeosciences, 20, 2347–2367, https://doi.org/10.5194/bg-20-2347-2023,https://doi.org/10.5194/bg-20-2347-2023, 2023

Short summary

Interdisciplinary strategy to assess the impact of meteorological variables on the biochemical composition of the rain and the dynamics of a small eutrophic lake under rain forcing

Fanny Noirmain, Jean-Luc Baray, Frédéric Tridon, Philippe Cacault, Hermine Billard, Guillaume Voyard, Joël Van Baelen, and Delphine Latour

Biogeosciences, 19, 5729–5749, https://doi.org/10.5194/bg-19-5729-2022,https://doi.org/10.5194/bg-19-5729-2022, 2022

Short summary

Cited articles

Akbari, A., Wang, Z., He, P., Wang, D., Lee, J., Han, I. L., Li, G., and Gu, A. Z.: Unrevealed roles of polyphosphate-accumulating microorganisms, Microb. Biotechnol., 14, 82–87, https://doi.org/10.1111/1751-7915.13730, 2021.

Allan, R. A. and Miller, J. J.: Influence of S-adenosylmethionine on DAPI-induced fluorescence of polyphosphate in the yeast vacuole, Can. J. Microbiol., 26, 912–920, https://doi.org/10.1139/m80-158, 1980.

Amini, M., Antelo, J., Fiol, S., and Rahnemaie, R.: Estimation of phosphate extractability in flooded soils: Effect of solid-solution ratio and bicarbonate concentration, Chemosphere, 303, 135188, https://doi.org/10.1016/j.chemosphere.2022.135188, 2022.

Angelova, P. R., Agrawalla, B. K., Elustondo, P. A., Gordon, J., Shiba, T., Abramov, A. Y., Chang, Y.-T., and Pavlov, E. V.: In situ investigation of mammalian inorganic polyphosphate localization using novel selective fluorescent probes JC-D7 and JC-D8, ACS Chem. Biol., 9, 2101–2110, https://doi.org/10.1021/cb5000696, 2014.

Aschar-Sobbi, R., Abramov, A. Y., Diao, C., Kargacin, M. E., Kargacin, G. J., French, R. J., and Pavlov, E.: High sensitivity, quantitative measurements of polyphosphate using a new DAPI-based approach, J. Fluoresc., 18, 859–866, https://doi.org/10.1007/s10895-008-0315-4, 2008.