Articles | Volume 22, issue 17
https://doi.org/10.5194/bg-22-4579-2025
https://doi.org/10.5194/bg-22-4579-2025
Research article
 | 
12 Sep 2025
Research article |  | 12 Sep 2025

Variability in oxygen isotopic fractionation of enzymatic O2 consumption

Carolina F. M. de Carvalho, Moritz F. Lehmann, and Sarah G. Pati

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Cited articles

Ash, J. L., Hu, H., and Yeung, L. Y.: What fractionates oxygen isotopes during respiration? Insights from multiple isotopologue measurements and theory, ACS Earth and Space Chemistry, 4, 50–66, https://doi.org/10.1021/acsearthspacechem.9b00230, 2020. 
Bauer, J. A., Zámocká, M., Majtán, J., and Bauerová-Hlinková, V.: Glucose oxidase, an enzyme “Ferrari”: Its structure, function, production and properties in the light of various industrial and biotechnological applications, Biomolecules, 12, 472, 2022. 
Bender, M. L.: The δ18O of dissolved O2 in seawater: A unique tracer of circulation and respiration in the deep sea, J. Geophys. Res., 95, 22243–22252, https://doi.org/10.1029/JC095iC12p22243, 1990. 
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Using O2 stable isotope analysis, we determined the isotopic fractionation of biological O2 consumption by 10 flavin-dependent enzymes and 6 metalloenzymes. Metalloenzymes displayed a narrower range and lower values of isotopic fractionation than flavin-dependent enzymes. This work expands our understanding of the variability of oxygen isotopic fractionation at the enzyme level, improving the ability to study O2 dynamics from molecular to ecosystem scales.
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