Biogeosciences
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Articles | Volume 22, issue 17
Articles | Volume 22, issue 17
https://doi.org/10.5194/bg-22-4579-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-22-4579-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 22, issue 17
Research article
 | 
12 Sep 2025
Research article |  | 12 Sep 2025

Variability in oxygen isotopic fractionation of enzymatic O2 consumption

Carolina F. M. de Carvalho, Moritz F. Lehmann, and Sarah G. Pati

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Short summary
Using O2 stable isotope analysis, we determined the isotopic fractionation of biological O2 consumption by 10 flavin-dependent enzymes and 6 metalloenzymes. Metalloenzymes displayed a narrower range and lower values of isotopic fractionation than flavin-dependent enzymes. This work expands our understanding of the variability of oxygen isotopic fractionation at the enzyme level, improving the ability to study O2 dynamics from molecular to ecosystem scales.
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