Symbiotic ectomycorrhizal (ECM) fungi have an important role in mineral weathering and base cation uptake in boreal forests. We characterised base cation transporter diversity and their evolution in ECM fungi in relation to weathering capabilities, and quantified base cation uptake from pulverised rock. Base cation transporter gene families evolved rapidly in the genus Suillus, with gene copy numbers correlating with base cation uptake in some cases, suggesting links to weathering capabilities.