Articles | Volume 23, issue 4
https://doi.org/10.5194/bg-23-1591-2026
https://doi.org/10.5194/bg-23-1591-2026
Research article
 | 
27 Feb 2026
Research article |  | 27 Feb 2026

A simplified approach for measuring Rubisco carbon isotope fractionation and the first determination in marine haptophyte Gephyrocapsa oceanica

Reto S. Wijker, Pere Aguiló-Nicolau, Madalina Jaggi, Jeroni Galmés, and Heather M. Stoll

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This preprint is open for discussion and under review for Climate of the Past (CP).
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Cited articles

Aguiló-Nicolau, P., Galmés, J., Fais, G., Capó-Bauçà, S., Cao, G., and Iñiguez, C.: Singular adaptations in the carbon assimilation mechanism of the polyextremophile cyanobacterium Chroococcidiopsis thermalis, Photosynthesis Research, 156, 231–245, https://doi.org/10.1007/s11120-023-01008-y, 2023. a
Aguiló Nicolau, P., Wijker, R. S., Iñiguez, C., Capó-Bauçà, S., Stoll, H. M., and Galmés, J.: Diversity in rubisco kinetics and CO2-concentrating mechanisms among cyanobacterial lineages, bioRxiv [preprint], https://doi.org/10.64898/2025.12.30.696979, 2025. a, b, c, d, e
Amaral, J., Lobo, A. K. M., Carmo-Silva, E., and Orr, D. J.: Purification of Rubisco from Leaves, Springer US, New York, NY, 417–426, ISBN 978-1-0716-3790-6, https://doi.org/10.1007/978-1-0716-3790-6_22, 2024. a
Banda, D. M., Pereira, J. H., Liu, A. K., Orr, D. J., Hammel, M., He, C., Parry, M. A. J., Carmo-Silva, E., Adams, P. D., Banfield, J. F., and Shih, P. M.: Novel bacterial clade reveals origin of form I Rubisco, Nature Plants, 6, 1158–1166, https://doi.org/10.1038/s41477-020-00762-4, 2020. a
Bar-On, Y. M. and Milo, R.: The global mass and average rate of rubisco, Proceedings of the National Academy of Sciences, 116, 4738–4743, https://doi.org/10.1073/pnas.1816654116, 2019. a
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Photosynthetic organisms leave characteristic carbon isotope fingerprints that help scientists study Earth’s carbon cycle. We developed a simpler laboratory method to measure these isotope effects in Rubisco, the key enzyme fixing CO2. Applying this approach, we obtained the first measurement for Gephyrocapsa oceanica, a widespread marine alga that plays a major role in ocean carbon cycling.
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