Particles under stress: ultrasonication causes size and recovery rate artifacts with soil-derived POM but not with microplastics

Büks, Frederick; Kayser, Gilles; Zieger, Antonia; Lang, Friederike; Kaupenjohann, Martin

doi:https://doi.org/10.5194/bg-18-159-2021

Articles | Volume 18, issue 1

https://doi.org/10.5194/bg-18-159-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/bg-18-159-2021

© Author(s) 2021. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 18, issue 1

Research article

|

11 Jan 2021

Research article |

| 11 Jan 2021

Particles under stress: ultrasonication causes size and recovery rate artifacts with soil-derived POM but not with microplastics

Frederick Büks, Gilles Kayser, Antonia Zieger, Friederike Lang, and Martin Kaupenjohann

Download

Final revised paper (published on 11 Jan 2021)
Supplement to the final revised paper
Preprint (discussion started on 16 Jun 2020)
Supplement to the preprint

Interactive discussion

Status: closed

AC: Author comment | RC: Referee comment | SC: Short comment | EC: Editor comment

- Printer-friendly version

- Supplement

RC1: 'Review', Anonymous Referee #1, 30 Jun 2020
- AC1: 'Reply to referee #1', Frederick Büks, 16 Jul 2020
RC2: 'Referee 2', Anonymous Referee #2, 14 Sep 2020
- AC2: 'Reply to referee #2', Frederick Büks, 23 Oct 2020

Peer-review completion

AR: Author's response | RR: Referee report | ED: Editor decision

ED: Reconsider after major revisions (29 Oct 2020) by Yakov Kuzyakov

AR by Frederick Büks on behalf of the Authors (02 Nov 2020) Author's response Manuscript

ED: Publish as is (21 Nov 2020) by Yakov Kuzyakov

AR by Frederick Büks on behalf of the Authors (23 Nov 2020) Author's response Manuscript

Download

Article (1687 KB)
Full-text XML

Short summary

Ultrasonication/density fractionation is a common method used to extract particulate organic matter (POM) and, recently, microplastic (MP) from soil samples. In this study, ultrasonic treatment with mechanical stress increasing from 0 to 500 J mL⁻¹ caused comminution and a reduced recovery rate of soil-derived POMs but no such effects with MP particles. In consequence, the extraction of MP from soils is not affected by particle size and recovery rate artifacts.