Biogeosciences
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Articles | Volume 22, issue 21
Articles | Volume 22, issue 21
https://doi.org/10.5194/bg-22-6225-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-22-6225-2025
© Author(s) 2025. This work is distributed under
the Creative Commons Attribution 4.0 License.
Articles | Volume 22, issue 21
Research article
 | 
30 Oct 2025
Research article |  | 30 Oct 2025

Respiration rates of marine prokaryotes and implications for the in vivo INT method

Isabel Seguro, Kevin Vikström, Jonathan D. Todd, Stephen J. Giovannoni, E. Elena García-Martín, Robert Utting, and Carol Robinson

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Short summary
This study is the first to test the iodonitrotetrazolium reduction (INTR) method for respiration in prokaryotes. The method was successfully used with γ-proteobacteria, α-proteobacteria (including SAR11), actinobacteria and cyanobacteria. We show that the current use of a single conversion from INTR to oxygen consumption may not be universally applicable but should be determined for each community. The results provide further confidence in the INT method to determine plankton respiration.
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