Articles | Volume 22, issue 21
https://doi.org/10.5194/bg-22-6275-2025
https://doi.org/10.5194/bg-22-6275-2025
Research article
 | 
30 Oct 2025
Research article |  | 30 Oct 2025

On-site microbiome study of silica structures in a subterranean Mars analog environment

Martina Cappelletti, Giacomo Broglia, Andrea Firrincieli, Ettore Lopo, Alice Checcucci, Daniele Ghezzi, Federico Pisani, Freddy Vergara, Bruno Casarotto, and Francesco Sauro

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Short summary
In this work, we developed, applied and validated on-site microbiological procedures to study the unique silica stromatolite-like structures present in orthoquartzite caves on venezuelan tepuis. The results prove them as promising terrestrial astrobiological analogues, validate the feasibility of real-time microbial analyses in remote caves and provide novel understanding on the microbiological aspects involved in the formation of silica stromatolites in non-thermal and aphotic environments.
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