Precision of phytoplankton pigment analysis by high-performance liquid chromatography: an assessment of the global ocean color validation dataset analyzed by NASA

Chaves, Joaquín E.; Thomas, Crystal S.; Mannino, Antonio

doi:10.5194/bg-23-7043-2026

Articles | Volume 23, issue 19

https://doi.org/10.5194/bg-23-7043-2026

© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.

https://doi.org/10.5194/bg-23-7043-2026

© Author(s) 2026. This work is distributed under
the Creative Commons Attribution 4.0 License.

Articles | Volume 23, issue 19

Research article

|

08 Oct 2026

Research article |

| 08 Oct 2026

Precision of phytoplankton pigment analysis by high-performance liquid chromatography: an assessment of the global ocean color validation dataset analyzed by NASA

Joaquín E. Chaves, Crystal S. Thomas, and Antonio Mannino

Related authors

A compilation of global bio-optical in situ data for ocean-colour satellite applications – version four

Salem Ibrahim Salem, Andre Valente, Shubha Sathyendranath, Andrei Chuprin, Théo Renard, Jaime Pitarch, Ruth Airs, Moreno Ana, Maria-Manuel Angelico, David Antoine, Robert Arnone, Florinda Artuso, Paulo O. Oliveira, William Balch, Kathryn Barker, Ray Barlow, Simon Bélanger, Marco Bellacicco, Jean-François Berthon, Şükrü Beşiktepe, Francesco Bignami, Yngve Borsheim, Astrid Bracher, Federica Braga, Vittorio Brando, Robert J. W. Brewin, Vanda Brotas, Elisabetta Canuti, Francisco P. Chavez, Andrés Cianca, Hervé Claustre, Lesley Clementson, Simone Colella, Raul Costa, Cristina Nunes, Richard Crout, Emmanuel Devred, Annalisa Di Cicco, Federico Falcini, Ivan Farace, Afonso Ferreira, Scott Freeman, Robert Frouin, Carlos García-Soto, Isabella Giardina, Stuart W. Gibb, Arthur Gleason, Ralf Goericke, Richard Gould, Michael Grant, Nathalie Guillocheau, Hiroto Higa, Stanford B. Hooker, Chuamin Hu, Joji Ishizaka, Thomas Jackson, Mati Kahru, Milton Kampel, Bokyung Kim, Holger Klein, Piotr Kowalczuk, Susanne Kratzer, Raphael Kudela, Antonia Lai, Rosario Lavezza, Jesus Ledesma, Huiwon Lee, Steven Lohrenz, Hubert Loisel, Vivian Lutz, Antonio Mannino, Francesca Margiotta, Victor Martinez-Vicente, Salvatore Marullo, Luca Massi, Patricia Matrai, Atsushi Matsuoka, David McKee, Chiara Melillo, Carlos B. Mendes, Brian G. Mitchell, Tiffany Moisan, Enrique Montes, Frank Muller-Karger, Aimee Neeley, Michael Novak, Leonie O'Dowd, Michael Ondrusek, Emanuele Organelli, Heeyoon Park, Trevor Platt, Francesca Polonelli, Alex Poulton, Mariella Ragni, Michel Repecaud, Maurizio Ribera d’Alcalà, Tamara Rodriguez-Ramos, Rüdiger Röttgers, Manuel Ruiz-Villarreal, Michela Sammartino, Rosalia Santoleri, Gian Marco Scarpa, Thomas Schroeder, Valeria Segura, Timothy Smyth, Denise Smythe-Wright, Heidi M. Sosik, Joanna Stoń-Egiert, Malcolm Taberner, Jing Tan, Rob Thomas, Crystal Thomas, Gavin Tilstone, Andreia Tracana, Michael Twardowski, Vincenzo Vellucci, Gianluca Volpe, Kenneth Voss, Jeremy Werdell, Marcel Wernand, Bozena Wojtasiewicz, Simon Wright, Sunmi Yang, Giuseppe Zibordi, Maria Laura Zoffoli, and Steve Groom

Earth Syst. Sci. Data Discuss., https://doi.org/10.5194/essd-2026-539,https://doi.org/10.5194/essd-2026-539, 2026

Preprint under review for ESSD

Short summary

A compilation of surface inherent optical properties and phytoplankton pigment concentrations from the Atlantic Meridional Transect

Thomas M. Jordan, Giorgio Dall'Olmo, Gavin Tilstone, Robert J. W. Brewin, Francesco Nencioli, Ruth Airs, Crystal S. Thomas, and Louise Schlüter

Earth Syst. Sci. Data, 17, 493–516, https://doi.org/10.5194/essd-17-493-2025,https://doi.org/10.5194/essd-17-493-2025, 2025

Short summary

Nunataryuk field campaigns: understanding the origin and fate of terrestrial organic matter in the coastal waters of the Mackenzie Delta region

Martine Lizotte, Bennet Juhls, Atsushi Matsuoka, Philippe Massicotte, Gaëlle Mével, David Obie James Anikina, Sofia Antonova, Guislain Bécu, Marine Béguin, Simon Bélanger, Thomas Bossé-Demers, Lisa Bröder, Flavienne Bruyant, Gwénaëlle Chaillou, Jérôme Comte, Raoul-Marie Couture, Emmanuel Devred, Gabrièle Deslongchamps, Thibaud Dezutter, Miles Dillon, David Doxaran, Aude Flamand, Frank Fell, Joannie Ferland, Marie-Hélène Forget, Michael Fritz, Thomas J. Gordon, Caroline Guilmette, Andrea Hilborn, Rachel Hussherr, Charlotte Irish, Fabien Joux, Lauren Kipp, Audrey Laberge-Carignan, Hugues Lantuit, Edouard Leymarie, Antonio Mannino, Juliette Maury, Paul Overduin, Laurent Oziel, Colin Stedmon, Crystal Thomas, Lucas Tisserand, Jean-Éric Tremblay, Jorien Vonk, Dustin Whalen, and Marcel Babin

Earth Syst. Sci. Data, 15, 1617–1653, https://doi.org/10.5194/essd-15-1617-2023,https://doi.org/10.5194/essd-15-1617-2023, 2023

Short summary

A compilation of global bio-optical in situ data for ocean colour satellite applications – version three

André Valente, Shubha Sathyendranath, Vanda Brotas, Steve Groom, Michael Grant, Thomas Jackson, Andrei Chuprin, Malcolm Taberner, Ruth Airs, David Antoine, Robert Arnone, William M. Balch, Kathryn Barker, Ray Barlow, Simon Bélanger, Jean-François Berthon, Şükrü Beşiktepe, Yngve Borsheim, Astrid Bracher, Vittorio Brando, Robert J. W. Brewin, Elisabetta Canuti, Francisco P. Chavez, Andrés Cianca, Hervé Claustre, Lesley Clementson, Richard Crout, Afonso Ferreira, Scott Freeman, Robert Frouin, Carlos García-Soto, Stuart W. Gibb, Ralf Goericke, Richard Gould, Nathalie Guillocheau, Stanford B. Hooker, Chuamin Hu, Mati Kahru, Milton Kampel, Holger Klein, Susanne Kratzer, Raphael Kudela, Jesus Ledesma, Steven Lohrenz, Hubert Loisel, Antonio Mannino, Victor Martinez-Vicente, Patricia Matrai, David McKee, Brian G. Mitchell, Tiffany Moisan, Enrique Montes, Frank Muller-Karger, Aimee Neeley, Michael Novak, Leonie O'Dowd, Michael Ondrusek, Trevor Platt, Alex J. Poulton, Michel Repecaud, Rüdiger Röttgers, Thomas Schroeder, Timothy Smyth, Denise Smythe-Wright, Heidi M. Sosik, Crystal Thomas, Rob Thomas, Gavin Tilstone, Andreia Tracana, Michael Twardowski, Vincenzo Vellucci, Kenneth Voss, Jeremy Werdell, Marcel Wernand, Bozena Wojtasiewicz, Simon Wright, and Giuseppe Zibordi

Earth Syst. Sci. Data, 14, 5737–5770, https://doi.org/10.5194/essd-14-5737-2022,https://doi.org/10.5194/essd-14-5737-2022, 2022

Short summary

Cited articles

Araujo, P.: Key aspects of analytical method validation and linearity evaluation, METHOD Valid. Comp. Transf., 877, 2224–2234, https://doi.org/10.1016/j.jchromb.2008.09.030, 2009.

Behrenfeld, M. J., Randerson, J. T., McClain, C. R., Feldman, G. C., Los, S. O., Tucker, C. J., Falkowski, P. G., Field, C. B., Frouin, R., Esaias, W. E., Kolber, D. D., and Pollack, N. H.: Biospheric Primary Production During an ENSO Transition, Science, 291, 2594, https://doi.org/10.1126/science.1055071, 2001.

Behrenfeld, M. J., Westberry, T. K., Boss, E. S., O'Malley, R. T., Siegel, D. A., Wiggert, J. D., Franz, B. A., McClain, C. R., Feldman, G. C., Doney, S. C., Moore, J. K., Dall'Olmo, G., Milligan, A. J., Lima, I., and Mahowald, N.: Satellite-detected fluorescence reveals global physiology of ocean phytoplankton, Biogeosciences, 6, 779–794, https://doi.org/10.5194/bg-6-779-2009, 2009.

Bidigare, R. R., Van Heukelem, L. V., Trees, C. C., and Perl, J.: HPLC Phytoplankton Pigments: Sampling, Laboratory Methods, and Quality Assurance Procedures, in: Ocean Optics Protocols For Satellite Ocean Color Sensor Validation, vol. Revision 5, Volume V, edited by: Mueller, J. L., Fargion, G. S., and McClain, C. R., National Aeronautics and Space Administration, Greenbelt, Maryland, 20771, https://seabass.gsfc.nasa.gov/wiki/System_Description/Protocols_Ver5_VolV.pdf (last access: 11 March 2026), 2003.

Canjura, F. L.: Separation of chlorophyll compounds and their polar derivatives by high-performance liquid chromatography, J. Agric. Food Chem., 39, 1102–1105, https://doi.org/10.1021/jf00006a020, 1991.