Articles | Volume 20, issue 7
https://doi.org/10.5194/bg-20-1277-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
https://doi.org/10.5194/bg-20-1277-2023
© Author(s) 2023. This work is distributed under
the Creative Commons Attribution 4.0 License.
the Creative Commons Attribution 4.0 License.
Research article
| 
05 Apr 2023
Research article |
| 05 Apr 2023
| 05 Apr 2023
Ecological divergence of a mesocosm in an eastern boundary upwelling system assessed with multi-marker environmental DNA metabarcoding
Markus A. Min
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, USA
David M. Needham
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany
Sebastian Sudek
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
Nathan Kobun Truelove
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
Kathleen J. Pitz
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
Gabriela M. Chavez
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany
Camille Poirier
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany
Bente Gardeler
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany
Elisabeth von der Esch
Institute of Hydrochemistry, Technical University of Munich, Munich, Germany
Andrea Ludwig
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany
Ulf Riebesell
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany
Alexandra Z. Worden
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel, Germany
Monterey Bay Aquarium Research Institute, Moss Landing, CA, USA
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Salem Ibrahim Salem, Andre Valente, Shubha Sathyendranath, Andrei Chuprin, Théo Renard, Jaime Pitarch, Ruth Airs, Moreno Ana, Maria-Manuel Angelico, David Antoine, Robert Arnone, Florinda Artuso, Paulo O. Oliveira, William Balch, Kathryn Barker, Ray Barlow, Simon Bélanger, Marco Bellacicco, Jean-François Berthon, Şükrü Beşiktepe, Francesco Bignami, Yngve Borsheim, Astrid Bracher, Federica Braga, Vittorio Brando, Robert J. W. Brewin, Vanda Brotas, Elisabetta Canuti, Francisco P. Chavez, Andrés Cianca, Hervé Claustre, Lesley Clementson, Simone Colella, Raul Costa, Cristina Nunes, Richard Crout, Emmanuel Devred, Annalisa Di Cicco, Federico Falcini, Ivan Farace, Afonso Ferreira, Scott Freeman, Robert Frouin, Carlos García-Soto, Isabella Giardina, Stuart W. Gibb, Arthur Gleason, Ralf Goericke, Richard Gould, Michael Grant, Nathalie Guillocheau, Hiroto Higa, Stanford B. Hooker, Chuamin Hu, Joji Ishizaka, Thomas Jackson, Mati Kahru, Milton Kampel, Bokyung Kim, Holger Klein, Piotr Kowalczuk, Susanne Kratzer, Raphael Kudela, Antonia Lai, Rosario Lavezza, Jesus Ledesma, Huiwon Lee, Steven Lohrenz, Hubert Loisel, Vivian Lutz, Antonio Mannino, Francesca Margiotta, Victor Martinez-Vicente, Salvatore Marullo, Luca Massi, Patricia Matrai, Atsushi Matsuoka, David McKee, Chiara Melillo, Carlos B. Mendes, Brian G. Mitchell, Tiffany Moisan, Enrique Montes, Frank Muller-Karger, Aimee Neeley, Michael Novak, Leonie O'Dowd, Michael Ondrusek, Emanuele Organelli, Heeyoon Park, Trevor Platt, Francesca Polonelli, Alex Poulton, Mariella Ragni, Michel Repecaud, Maurizio Ribera d’Alcalà, Tamara Rodriguez-Ramos, Rüdiger Röttgers, Manuel Ruiz-Villarreal, Michela Sammartino, Rosalia Santoleri, Gian Marco Scarpa, Thomas Schroeder, Valeria Segura, Timothy Smyth, Denise Smythe-Wright, Heidi M. Sosik, Joanna Stoń-Egiert, Malcolm Taberner, Jing Tan, Rob Thomas, Crystal Thomas, Gavin Tilstone, Andreia Tracana, Michael Twardowski, Vincenzo Vellucci, Gianluca Volpe, Kenneth Voss, Jeremy Werdell, Marcel Wernand, Bozena Wojtasiewicz, Simon Wright, Sunmi Yang, Giuseppe Zibordi, Maria Laura Zoffoli, and Steve Groom
Earth Syst. Sci. Data Discuss., https://doi.org/10.5194/essd-2026-539, https://doi.org/10.5194/essd-2026-539, 2026
Preprint under review for ESSD
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This paper presents the fourth version of the ESA Ocean Colour Climate Change Initiative global in situ bio-optical database. The updated compilation combines observations from many international data sources collected between 1996 and 2025 and includes major expansions in coverage and variables. The dataset provides a consistent reference for validating satellite ocean-colour products and supports algorithm development and long-term climate studies.
Patricia Grasse, Kristin Doering, Allanah Joy Paul, Avy Bernales, Sonia Sanchez Ramirez, Elisabeth von der Esch, Michelle Graco, Tim Boxhammer, Lennart Thomas Bach, Ulf Riebesell, and Martin Frank
Biogeosciences, 23, 5515–5532, https://doi.org/10.5194/bg-23-5515-2026, https://doi.org/10.5194/bg-23-5515-2026, 2026
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Mesocosm experiments in the Peruvian Upwelling during a coastal El Niño examined silicon cycling and isotope dynamics under changing nutrient conditions. The addition of nitrate-depleted deep water shifted the phytoplankton community from diatoms to silicoflagellates, altering Si isotope signatures. We provide the first field-based estimate of the Si isotope fractionation factor for silicoflagellates, showing their potential importance for interpreting modern and past marine silicon cycling.
Philipp Suessle, Kai Georg Schulz, Joana Barcelos e Ramos, Nico Manuel Sievers, Julieta Schneider, Juliane Katharina Tammen, Leila Kittu, Laura Marín-Samper, Maarten Boersma, and Ulf Riebesell
Biogeosciences, 23, 4691–4710, https://doi.org/10.5194/bg-23-4691-2026, https://doi.org/10.5194/bg-23-4691-2026, 2026
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Ocean alkalinity enhancement (OAE) is a negative emission technology that may alter marine diatom communities and particle export. We show that unequilibrated OAE did not change bloom magnitude or particle fluxes, but reduced silica ballasting. The losses intensified during sinking, consistent with pH-enhanced silica dissolution. Our results show that OAE can modify biological-pump efficiency through export-phase alteration of BSi:POC, even when community biomass production remains unchanged.
Julieta Schneider, Ulf Riebesell, Charly André Moras, Laura Marín-Samper, Leila Richards Kittu, Joaquín Ortíz-Cortes, and Kai Georg Schulz
Biogeosciences, 23, 137–153, https://doi.org/10.5194/bg-23-137-2026, https://doi.org/10.5194/bg-23-137-2026, 2026
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Ocean Alkalinity Enhancement (OAE) is an approach to sequester atmospheric CO2 in the ocean and may alleviate ocean acidification. A large-scale mesocosm experiment in Norway tested calcium- and silicate-based OAE, increasing total alkalinity (TA) by 0–600 µmol kg-1 and measuring CO2 gas exchange. While TA remained stable, we found mineral-type and/or pCO2/pH effects on coccolithophorid calcification, net community production and zooplankton respiration, providing insights for future OAE trials.
Allanah Joy Paul, Mathias Haunost, Silvan Urs Goldenberg, Jens Hartmann, Nicolás Sánchez, Julieta Schneider, Niels Suitner, and Ulf Riebesell
Biogeosciences, 22, 2749–2766, https://doi.org/10.5194/bg-22-2749-2025, https://doi.org/10.5194/bg-22-2749-2025, 2025
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Ocean alkalinity enhancement (OAE) is being assessed for its potential to absorb atmospheric CO2 and store it for a long time. OAE still needs comprehensive assessment of its safety and effectiveness. We studied an idealised OAE application in a natural low-nutrient ecosystem over 1 month. Our results showed that biogeochemical functioning remained mostly stable but that the long-term capability for storing carbon may be limited at high alkalinity concentration.
Ulf Riebesell
Biogeosciences, 22, 2381–2381, https://doi.org/10.5194/bg-22-2381-2025, https://doi.org/10.5194/bg-22-2381-2025, 2025
Librada Ramírez, Leonardo J. Pozzo-Pirotta, Aja Trebec, Víctor Manzanares-Vázquez, José L. Díez, Javier Arístegui, Ulf Riebesell, Stephen D. Archer, and María Segovia
Biogeosciences, 22, 1865–1886, https://doi.org/10.5194/bg-22-1865-2025, https://doi.org/10.5194/bg-22-1865-2025, 2025
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We studied the potential effects of increasing ocean alkalinity on a natural plankton community in subtropical waters of the Atlantic near Gran Canaria, Spain. Alkalinity is the capacity of water to resist acidification, and plankton are usually microscopic plants (phytoplankton) and animals (zooplankton), often less than 2.5 cm in length. This study suggests that increasing ocean alkalinity did not have a significant negative impact on the plankton community studied.
Giulia Faucher, Mathias Haunost, Allanah Joy Paul, Anne Ulrike Christiane Tietz, and Ulf Riebesell
Biogeosciences, 22, 405–415, https://doi.org/10.5194/bg-22-405-2025, https://doi.org/10.5194/bg-22-405-2025, 2025
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Ocean alkalinity enhancement (OAE) is being evaluated for its capacity to absorb atmospheric CO2 in the ocean and store it long term to mitigate climate change. As researchers plan for field tests to gain insights into OAE, sharing knowledge on its environmental impact on marine ecosystems is urgent. Our study examined NaOH-induced OAE in Emiliania huxleyi, a key coccolithophore species, and found that the added total alkalinity (ΔTA) should stay below 600 µmol kg⁻¹ to avoid negative impacts.
Philipp Suessle, Jan Taucher, Silvan Urs Goldenberg, Moritz Baumann, Kristian Spilling, Andrea Noche-Ferreira, Mari Vanharanta, and Ulf Riebesell
Biogeosciences, 22, 71–86, https://doi.org/10.5194/bg-22-71-2025, https://doi.org/10.5194/bg-22-71-2025, 2025
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Ocean alkalinity enhancement (OAE) is a negative emission technology which may alter marine communities and the particle export they drive. Here, impacts of carbonate-based OAE on the flux and attenuation of sinking particles in an oligotrophic plankton community are presented. Whilst biological parameters remained unaffected, abiotic carbonate precipitation occurred. Among counteracting OAE’s efficiency, it influenced mineral ballasting and particle sinking velocities, requiring monitoring.
Laura Marín-Samper, Javier Arístegui, Nauzet Hernández-Hernández, and Ulf Riebesell
Biogeosciences, 21, 5707–5724, https://doi.org/10.5194/bg-21-5707-2024, https://doi.org/10.5194/bg-21-5707-2024, 2024
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This study exposed a natural community to two non-CO2-equilibrated ocean alkalinity enhancement (OAE) deployments using different minerals. Adding alkalinity in this manner decreases dissolved CO2, essential for photosynthesis. While photosynthesis was not suppressed, bloom formation was mildly delayed, potentially impacting marine food webs. The study emphasizes the need for further research on OAE without prior equilibration and on its ecological implications.
Niels Suitner, Giulia Faucher, Carl Lim, Julieta Schneider, Charly A. Moras, Ulf Riebesell, and Jens Hartmann
Biogeosciences, 21, 4587–4604, https://doi.org/10.5194/bg-21-4587-2024, https://doi.org/10.5194/bg-21-4587-2024, 2024
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Recent studies described the precipitation of carbonates as a result of alkalinity enhancement in seawater, which could adversely affect the carbon sequestration potential of ocean alkalinity enhancement (OAE) approaches. By conducting experiments in natural seawater, this study observed uniform patterns during the triggered runaway carbonate precipitation, which allow the prediction of safe and efficient local application levels of OAE scenarios.
Silvan Urs Goldenberg, Ulf Riebesell, Daniel Brüggemann, Gregor Börner, Michael Sswat, Arild Folkvord, Maria Couret, Synne Spjelkavik, Nicolás Sánchez, Cornelia Jaspers, and Marta Moyano
Biogeosciences, 21, 4521–4532, https://doi.org/10.5194/bg-21-4521-2024, https://doi.org/10.5194/bg-21-4521-2024, 2024
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Ocean alkalinity enhancement (OAE) is being evaluated as a carbon dioxide removal technology for climate change mitigation. With an experiment on species communities, we show that larval and juvenile fish can be resilient to the resulting perturbation of seawater. Fish may hence recruit successfully and continue to support fisheries' production in regions of OAE. Our findings help to establish an environmentally safe operating space for this ocean-based solution.
Sebastian I. Cantarero, Edgart Flores, Harry Allbrook, Paulina Aguayo, Cristian A. Vargas, John E. Tamanaha, J. Bentley C. Scholz, Lennart T. Bach, Carolin R. Löscher, Ulf Riebesell, Balaji Rajagopalan, Nadia Dildar, and Julio Sepúlveda
Biogeosciences, 21, 3927–3958, https://doi.org/10.5194/bg-21-3927-2024, https://doi.org/10.5194/bg-21-3927-2024, 2024
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Our study explores lipid remodeling in response to environmental stress, specifically how cell membrane chemistry changes. We focus on intact polar lipids in a phytoplankton community exposed to diverse stressors in a mesocosm experiment. The observed remodeling indicates acyl chain recycling for energy storage in intact polar lipids during stress, reallocating resources based on varying growth conditions. This understanding is essential to grasp the system's impact on cellular pools.
Laura Marín-Samper, Javier Arístegui, Nauzet Hernández-Hernández, Joaquín Ortiz, Stephen D. Archer, Andrea Ludwig, and Ulf Riebesell
Biogeosciences, 21, 2859–2876, https://doi.org/10.5194/bg-21-2859-2024, https://doi.org/10.5194/bg-21-2859-2024, 2024
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Our planet is facing a climate crisis. Scientists are working on innovative solutions that will aid in capturing the hard to abate emissions before it is too late. Exciting research reveals that ocean alkalinity enhancement, a key climate change mitigation strategy, does not harm phytoplankton, the cornerstone of marine ecosystems. Through meticulous study, we may have uncovered a positive relationship: up to a specific limit, enhancing ocean alkalinity boosts photosynthesis by certain species.
Aaron Ferderer, Kai G. Schulz, Ulf Riebesell, Kirralee G. Baker, Zanna Chase, and Lennart T. Bach
Biogeosciences, 21, 2777–2794, https://doi.org/10.5194/bg-21-2777-2024, https://doi.org/10.5194/bg-21-2777-2024, 2024
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Ocean alkalinity enhancement (OAE) is a promising method of atmospheric carbon removal; however, its ecological impacts remain largely unknown. We assessed the effects of simulated silicate- and calcium-based mineral OAE on diatom silicification. We found that increased silicate concentrations from silicate-based OAE increased diatom silicification. In contrast, the enhancement of alkalinity had no effect on community silicification and minimal effects on the silicification of different genera.
David González-Santana, María Segovia, Melchor González-Dávila, Librada Ramírez, Aridane G. González, Leonardo J. Pozzo-Pirotta, Veronica Arnone, Victor Vázquez, Ulf Riebesell, and J. Magdalena Santana-Casiano
Biogeosciences, 21, 2705–2715, https://doi.org/10.5194/bg-21-2705-2024, https://doi.org/10.5194/bg-21-2705-2024, 2024
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In a recent experiment off the coast of Gran Canaria (Spain), scientists explored a method called ocean alkalinization enhancement (OAE), where carbonate minerals were added to seawater. This process changed the levels of certain ions in the water, affecting its pH and buffering capacity. The researchers were particularly interested in how this could impact the levels of essential trace metals in the water.
Xiaoke Xin, Giulia Faucher, and Ulf Riebesell
Biogeosciences, 21, 761–772, https://doi.org/10.5194/bg-21-761-2024, https://doi.org/10.5194/bg-21-761-2024, 2024
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Ocean alkalinity enhancement (OAE) is a promising approach to remove CO2 by accelerating natural rock weathering. However, some of the alkaline substances contain trace metals which could be toxic to marine life. By exposing three representative phytoplankton species to Ni released from alkaline materials, we observed varying responses of phytoplankton to nickel concentrations, suggesting caution should be taken and toxic thresholds should be avoided in OAE with Ni-rich materials.
Ulf Riebesell, Daniela Basso, Sonja Geilert, Andrew W. Dale, and Matthias Kreuzburg
State Planet, 2-oae2023, 6, https://doi.org/10.5194/sp-2-oae2023-6-2023, https://doi.org/10.5194/sp-2-oae2023-6-2023, 2023
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Mesocosm experiments represent a highly valuable tool in determining the safe operating space of ocean alkalinity enhancement (OAE) applications. By combining realism and biological complexity with controllability and replication, they provide an ideal OAE test bed and a critical stepping stone towards field applications. Mesocosm approaches can also be helpful in testing the efficacy, efficiency and permanence of OAE applications.
Moritz Baumann, Allanah Joy Paul, Jan Taucher, Lennart Thomas Bach, Silvan Goldenberg, Paul Stange, Fabrizio Minutolo, and Ulf Riebesell
Biogeosciences, 20, 2595–2612, https://doi.org/10.5194/bg-20-2595-2023, https://doi.org/10.5194/bg-20-2595-2023, 2023
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The sinking velocity of marine particles affects how much atmospheric CO2 is stored inside our oceans. We measured particle sinking velocities in the Peruvian upwelling system and assessed their physical and biochemical drivers. We found that sinking velocity was mainly influenced by particle size and porosity, while ballasting minerals played only a minor role. Our findings help us to better understand the particle sinking dynamics in this highly productive marine system.
Kristian Spilling, Jonna Piiparinen, Eric P. Achterberg, Javier Arístegui, Lennart T. Bach, Maria T. Camarena-Gómez, Elisabeth von der Esch, Martin A. Fischer, Markel Gómez-Letona, Nauzet Hernández-Hernández, Judith Meyer, Ruth A. Schmitz, and Ulf Riebesell
Biogeosciences, 20, 1605–1619, https://doi.org/10.5194/bg-20-1605-2023, https://doi.org/10.5194/bg-20-1605-2023, 2023
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We carried out an enclosure experiment using surface water off Peru with different additions of oxygen minimum zone water. In this paper, we report on enzyme activity and provide data on the decomposition of organic matter. We found very high activity with respect to an enzyme breaking down protein, suggesting that this is important for nutrient recycling both at present and in the future ocean.
Jens Hartmann, Niels Suitner, Carl Lim, Julieta Schneider, Laura Marín-Samper, Javier Arístegui, Phil Renforth, Jan Taucher, and Ulf Riebesell
Biogeosciences, 20, 781–802, https://doi.org/10.5194/bg-20-781-2023, https://doi.org/10.5194/bg-20-781-2023, 2023
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CO2 can be stored in the ocean via increasing alkalinity of ocean water. Alkalinity can be created via dissolution of alkaline materials, like limestone or soda. Presented research studies boundaries for increasing alkalinity in seawater. The best way to increase alkalinity was found using an equilibrated solution, for example as produced from reactors. Adding particles for dissolution into seawater on the other hand produces the risk of losing alkalinity and degassing of CO2 to the atmosphere.
André Valente, Shubha Sathyendranath, Vanda Brotas, Steve Groom, Michael Grant, Thomas Jackson, Andrei Chuprin, Malcolm Taberner, Ruth Airs, David Antoine, Robert Arnone, William M. Balch, Kathryn Barker, Ray Barlow, Simon Bélanger, Jean-François Berthon, Şükrü Beşiktepe, Yngve Borsheim, Astrid Bracher, Vittorio Brando, Robert J. W. Brewin, Elisabetta Canuti, Francisco P. Chavez, Andrés Cianca, Hervé Claustre, Lesley Clementson, Richard Crout, Afonso Ferreira, Scott Freeman, Robert Frouin, Carlos García-Soto, Stuart W. Gibb, Ralf Goericke, Richard Gould, Nathalie Guillocheau, Stanford B. Hooker, Chuamin Hu, Mati Kahru, Milton Kampel, Holger Klein, Susanne Kratzer, Raphael Kudela, Jesus Ledesma, Steven Lohrenz, Hubert Loisel, Antonio Mannino, Victor Martinez-Vicente, Patricia Matrai, David McKee, Brian G. Mitchell, Tiffany Moisan, Enrique Montes, Frank Muller-Karger, Aimee Neeley, Michael Novak, Leonie O'Dowd, Michael Ondrusek, Trevor Platt, Alex J. Poulton, Michel Repecaud, Rüdiger Röttgers, Thomas Schroeder, Timothy Smyth, Denise Smythe-Wright, Heidi M. Sosik, Crystal Thomas, Rob Thomas, Gavin Tilstone, Andreia Tracana, Michael Twardowski, Vincenzo Vellucci, Kenneth Voss, Jeremy Werdell, Marcel Wernand, Bozena Wojtasiewicz, Simon Wright, and Giuseppe Zibordi
Earth Syst. Sci. Data, 14, 5737–5770, https://doi.org/10.5194/essd-14-5737-2022, https://doi.org/10.5194/essd-14-5737-2022, 2022
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A compiled set of in situ data is vital to evaluate the quality of ocean-colour satellite data records. Here we describe the global compilation of bio-optical in situ data (spanning from 1997 to 2021) used for the validation of the ocean-colour products from the ESA Ocean Colour Climate Change Initiative (OC-CCI). The compilation merges and harmonizes several in situ data sources into a simple format that could be used directly for the evaluation of satellite-derived ocean-colour data.
Allanah Joy Paul, Lennart Thomas Bach, Javier Arístegui, Elisabeth von der Esch, Nauzet Hernández-Hernández, Jonna Piiparinen, Laura Ramajo, Kristian Spilling, and Ulf Riebesell
Biogeosciences, 19, 5911–5926, https://doi.org/10.5194/bg-19-5911-2022, https://doi.org/10.5194/bg-19-5911-2022, 2022
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We investigated how different deep water chemistry and biology modulate the response of surface phytoplankton communities to upwelling in the Peruvian coastal zone. Our results show that the most influential drivers were the ratio of inorganic nutrients (N : P) and the microbial community present in upwelling source water. These led to unexpected and variable development in the phytoplankton assemblage that could not be predicted by the amount of inorganic nutrients alone.
Shao-Min Chen, Ulf Riebesell, Kai G. Schulz, Elisabeth von der Esch, Eric P. Achterberg, and Lennart T. Bach
Biogeosciences, 19, 295–312, https://doi.org/10.5194/bg-19-295-2022, https://doi.org/10.5194/bg-19-295-2022, 2022
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Oxygen minimum zones in the ocean are characterized by enhanced carbon dioxide (CO2) levels and are being further acidified by increasing anthropogenic atmospheric CO2. Here we report CO2 system measurements in a mesocosm study offshore Peru during a rare coastal El Niño event to investigate how CO2 dynamics may respond to ongoing ocean deoxygenation. Our observations show that nitrogen limitation, productivity, and plankton community shift play an important role in driving the CO2 dynamics.
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Short summary
Emerging molecular methods provide new ways of understanding how marine communities respond to changes in ocean conditions. Here, environmental DNA was used to track the temporal evolution of biological communities in the Peruvian coastal upwelling system and in an adjacent enclosure where upwelling was simulated. We found that the two communities quickly diverged, with the open ocean being one found during upwelling and the enclosure evolving to one found under stratified conditions.
Emerging molecular methods provide new ways of understanding how marine communities respond to...
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