Articles | Volume 20, issue 7
https://doi.org/10.5194/bg-20-1491-2023
https://doi.org/10.5194/bg-20-1491-2023
Research article
 | 
14 Apr 2023
Research article |  | 14 Apr 2023

Impact of metabolism and temperature on 2H ∕ 1H fractionation in lipids of the marine bacterium Shewanella piezotolerans WP3

Xin Chen, Weishu Zhao, Liang Dong, Huahua Jian, Lewen Liang, Jing Wang, and Fengping Wang

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Cited articles

Chen, X., Liu, X., Jia, H., Jin, J., Kong, W., and Huang, Y.: Inverse hydrogen isotope fractionation indicates heterotrophic microbial production of long-chain n-alkyl lipids in desolate Antarctic ponds, Geobiology, 19, 394–404, 2021. 
Chen, X., Dong, L., Zhao, W., Jian, H., Wang, J., and Wang, F.: The effects of metabolism and temperature on carbon isotope composition of lipids in marine bacterium Shewanella piezotolerans WP3, Chem. Geol., 606, 120963, https://doi.org/10.1016/j.chemgeo.2022.120963, 2022. 
Dirghangi, S. S. and Pagani, M.: Hydrogen isotope fractionation during lipid biosynthesis by Haloarcula marismortui, Geochim. Cosmochim. Ac., 119, 381–390, 2013a. 
Dirghangi, S. S. and Pagani, M.: Hydrogen isotope fractionation during lipid biosynthesis by Tetrahymena thermophila, Org. Geochem., 64, 105–111, 2013b. 
Dufault-Thompson, K., Jian, H., Cheng, R., Li, J., Wang, F., and Zhang, Y.: A genome-scale model of Shewanella piezotolerans simulates mechanisms of metabolic diversity and energy conservation, Msystems, 2, e00165–00116, 2017. 
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Short summary
Here, we studied the effects of metabolism and growth temperature on 2H/1H fractionation between fatty acids and growth water (εFA/water) by Shewanella piezotolerans WP3. Our results show that the εFA/water values display considerable variations for cultures grown on different substrates. Combined with metabolic model analysis, our results indicate that the central metabolic pathways exert a fundamental effect on the hydrogen isotope composition of lipids in heterotrophs.
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