Articles | Volume 15, issue 5
Biogeosciences, 15, 1415–1424, 2018
https://doi.org/10.5194/bg-15-1415-2018

Special issue: Biological soil crusts and their role in biogeochemical processes...

Biogeosciences, 15, 1415–1424, 2018
https://doi.org/10.5194/bg-15-1415-2018

Research article 08 Mar 2018

Research article | 08 Mar 2018

Revisiting chlorophyll extraction methods in biological soil crusts – methodology for determination of chlorophyll a and chlorophyll a + b as compared to previous methods

Jennifer Caesar et al.

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In our study we analyzed the efficiency of different chlorophyll extraction solvents and investigated the effect of different preparatory steps to determine the optimal extraction method for biological soil crusts. Based on our results we confirm a DMSO-based chlorophyll extraction method without grinding pretreatment and suggest to insert an intermediate shaking step for complete chlorophyll extraction.
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