Articles | Volume 17, issue 22
Biogeosciences, 17, 5513–5537, 2020
Biogeosciences, 17, 5513–5537, 2020

Research article 14 Nov 2020

Research article | 14 Nov 2020

N2O isotope approaches for source partitioning of N2O production and estimation of N2O reduction – validation with the 15N gas-flux method in laboratory and field studies

Dominika Lewicka-Szczebak et al.

Related authors

Comparing modified substrate induced respiration with selective inhibition (SIRIN) and N2O isotope approaches to estimate fungal contribution to denitrification in three arable soils under anoxic conditions
Lena Rohe, Traute-Heidi Anderson, Heinz Flessa, Anette Giesemann, Dominika Lewicka-Szczebak, Nicole Wrage-Mönnig, and Reinhard Well
Biogeosciences Discuss.,,, 2020
Preprint under review for BG
Short summary
The 15N gas-flux method to determine N2 flux: a comparison of different tracer addition approaches
Dominika Lewicka-Szczebak and Reinhard Well
SOIL, 6, 145–152,,, 2020
Short summary
Underestimation of denitrification rates from field application of the 15N gas flux method and its correction by gas diffusion modelling
Reinhard Well, Martin Maier, Dominika Lewicka-Szczebak, Jan-Reent Köster, and Nicolas Ruoss
Biogeosciences, 16, 2233–2246,,, 2019
Short summary
Early season N2O emissions under variable water management in rice systems: source-partitioning emissions using isotope ratios along a depth profile
Elizabeth Verhoeven, Matti Barthel, Longfei Yu, Luisella Celi, Daniel Said-Pullicino, Steven Sleutel, Dominika Lewicka-Szczebak, Johan Six, and Charlotte Decock
Biogeosciences, 16, 383–408,,, 2019
Short summary
Effect of soil saturation on denitrification in a grassland soil
Laura Maritza Cardenas, Roland Bol, Dominika Lewicka-Szczebak, Andrew Stuart Gregory, Graham Peter Matthews, William Richard Whalley, Thomas Henry Misselbrook, David Scholefield, and Reinhard Well
Biogeosciences, 14, 4691–4710,,, 2017
Short summary

Related subject area

Biogeochemistry: Stable Isotopes & Other Tracers
Radium-228-derived ocean mixing and trace element inputs in the South Atlantic
Yu-Te Hsieh, Walter Geibert, E. Malcolm S. Woodward, Neil J. Wyatt, Maeve C. Lohan, Eric P. Achterberg, and Gideon M. Henderson
Biogeosciences, 18, 1645–1671,,, 2021
Short summary
Nitrogen isotopic fractionations during nitric oxide production in an agricultural soil
Zhongjie Yu and Emily M. Elliott
Biogeosciences, 18, 805–829,,, 2021
Short summary
Silicon uptake and isotope fractionation dynamics by crop species
Daniel A. Frick, Rainer Remus, Michael Sommer, Jürgen Augustin, Danuta Kaczorek, and Friedhelm von Blanckenburg
Biogeosciences, 17, 6475–6490,,, 2020
Short summary
Modern silicon dynamics of a small high-latitude subarctic lake
Petra Zahajská, Carolina Olid, Johanna Stadmark, Sherilyn C. Fritz, Sophie Opfergelt, and Daniel J. Conley
Biogeosciences Discuss.,,, 2020
Revised manuscript accepted for BG
Short summary
Barium stable isotopes as a fingerprint of biological cycling in the Amazon River basin
Quentin Charbonnier, Julien Bouchez, Jérôme Gaillardet, and Éric Gayer
Biogeosciences, 17, 5989–6015,,, 2020
Short summary

Cited articles

Aulakh, M. S., Doran, J. W., and Mosier, A. R.: Field-Evaluation of 4 Methods for Measuring Denitrification, Soil Sci. Soc. Am. J., 55, 1332–1338, 1991. 
Baily, A., Watson, C. J., Laughlin, R., Matthews, D., McGeough, K., and Jordan, P.: Use of the 15N gas flux method to measure the source and level of N2O and N2 emissions from grazed grassland, Nutr. Cycl. Agroecosys., 94, 287–298, 2012. 
Barford, C. C., Montoya, J. P., Altabet, M. A., and Mitchell, R.: Steady-state nitrogen isotope effects of N2 and N2O production in Paracoccus denitrificans, Appl. Environ. Microb., 65, 989–994, 1999. 
Baumgärtel, B. and Benke, M.: Düngeempfehlungen Stickstoff: Getreide, Raps, Hackfrüchte, Landwirtschaftskammer Niedersachsen – Geschäftsbereich Landwirtschaft, Hannover, 2009. 
Bergsma, T. T., Ostrom, N. E., Emmons, M., and Robertson, G. P.: Measuring simultaneous fluxes from soil of N2O and N2 in the field using the 15N-Gas “nonequilihrium” technique, Environ. Sci. Technol., 35, 4307–4312, 2001. 
Short summary
We present the first validation of N2O isotopic approaches for estimating N2O source pathways and N2O reduction. These approaches are widely used for tracing soil nitrogen cycling, but the results of these estimations are very uncertain. Here we report the results from parallel treatments allowing for precise validation of these approaches, and we propose the best strategies for results interpretation, including the new idea of an isotope model integrating three isotopic signatures of N2O.
Final-revised paper