Articles | Volume 12, issue 13
Research article 14 Jul 2015
Research article | 14 Jul 2015
The oxygen isotope composition of phosphate released from phytic acid by the activity of wheat and Aspergillus niger phytase
C. von Sperber et al.
No articles found.
Andre Baldermann, Oliver Wasser, Elshan Abdullayev, Stefano Bernasconi, Stefan Löhr, Klaus Wemmer, Werner E. Piller, Maxim Rudmin, and Sylvain Richoz
Clim. Past Discuss.,
Preprint under review for CPShort summary
We identified the provenance, (post)depositional history, weathering conditions and hydroclimate that formed the detrital and authigenic silicate minerals and soil carbonates of the Valley of Lakes sediments in Central Asia during the Cenozoic (~34 to 21 Ma). Aridification pulses in continental Central Asia coincide with marine glaciation events and are caused by Cenozoic climate forcing and the exhumation of the Tian Shan and Altai mountains, which reduced the moisture influx by westerly winds.
Alba Zappone, Antonio Pio Rinaldi, Melchior Grab, Quinn C. Wenning, Clément Roques, Claudio Madonna, Anne C. Obermann, Stefano M. Bernasconi, Matthias S. Brennwald, Rolf Kipfer, Florian Soom, Paul Cook, Yves Guglielmi, Christophe Nussbaum, Domenico Giardini, Marco Mazzotti, and Stefan Wiemer
Solid Earth, 12, 319–343,Short summary
The success of the geological storage of carbon dioxide is linked to the availability at depth of a capable reservoir and an impermeable caprock. The sealing capacity of the caprock is a key parameter for long-term CO2 containment. Faults crosscutting the caprock might represent preferential pathways for CO2 to escape. A decameter-scale experiment on injection in a fault, monitored by an integrated network of multiparamerter sensors, sheds light on the mobility of fluids within the fault.
Luca Smeraglia, Nathan Looser, Olivier Fabbri, Flavien Choulet, Marcel Guillong, and Stefano M. Bernasconi
Solid Earth Discuss.,
Preprint under review for SE
Philipp Baumann, Juhwan Lee, Emmanuel Frossard, Laurie Paule Schönholzer, Lucien Diby, Valérie Kouamé Hgaza, Delwende Innocent Kiba, Andrew Sila, Keith Sheperd, and Johan Six
Preprint under review for SOILShort summary
This work delivers openly accessible and validated calibrations for diagnosing 26 soil properties based on mid-infrared spectroscopy. These were developed for four regions in Burkina Faso and Ivory Coast, including 80 fields of smallholder farmers. The models can help to site-specifically and cost-efficiently monitor soil quality and fertility constraints to ameliorate soils and yields of yam or other staple crops in the four regions in between the humid forest and the northern Guinean savanna.
Thomas J. Leutert, Sevasti Modestou, Stefano M. Bernasconi, and A. Nele Meckler
Clim. Past Discuss.,
Preprint under review for CPShort summary
The Miocene climatic optimum associated with high atmospheric CO2 levels (~17–14 million years ago) was followed by a period of dramatic ice sheet growth on Antarctica. We present a clumped isotope-based bottom water temperature record from the Southern Ocean covering this key climate transition. Our record reveals warm conditions and a substantial cooling preceding the main ice volume increase, possibly caused by thresholds involved in ice growth and/or regional effects at our study site.
Jolanda E. Reusser, René Verel, Daniel Zindel, Emmanuel Frossard, and Timothy I. McLaren
Biogeosciences, 17, 5079–5095,Short summary
Inositol phosphates (IPs) are a major pool of organic P in soil. However, information on their diversity and abundance in soil is limited. We isolated IPs from soil and characterised them using solution nuclear magnetic resonance (NMR) spectroscopy. For the first time, we provide direct spectroscopic evidence for the existence of a multitude of lower-order IPs in soil extracts previously not detected with NMR. Our findings will help provide new insight into the cycling of IPs in ecosystems.
Annika Fiskal, Eva Anthamatten, Longhui Deng, Xingguo Han, Lorenzo Lagostina, Anja Michel, Rong Zhu, Nathalie Dubois, Carsten J. Schubert, Stefano M. Bernasconi, and Mark A. Lever
Revised manuscript under review for BG
Julian Helfenstein, Chiara Pistocchi, Astrid Oberson, Federica Tamburini, Daniel S. Goll, and Emmanuel Frossard
Biogeosciences, 17, 441–454,Short summary
In this article we provide estimates of mean residence times of phosphorus in inorganic soil phosphorus pools. These values improve our understanding of the dynamics of phosphorus cycling and can be used to improve global land surface models.
Catharina Simone Nisbeth, Federica Tamburini, Jacob Kidmose, Søren Jessen, and David William O'Connell
Hydrol. Earth Syst. Sci. Discuss.,
Preprint withdrawnShort summary
Phosphorus contamination frequently causes eutrophication of freshwater lakes. However it is often difficult to establish the origin of the contaminating phosphorus. This study aims to contribute to the development and improvement of a method for tracing phosphorus in the freshwater environment, by using the oxygen-18 isotope of orthophosphate (δ18Op). The use of a coherent and common method across research groups may enable phosphorus tracing and better management of freshwater ecosystems.
Annika Fiskal, Longhui Deng, Anja Michel, Philip Eickenbusch, Xingguo Han, Lorenzo Lagostina, Rong Zhu, Michael Sander, Martin H. Schroth, Stefano M. Bernasconi, Nathalie Dubois, and Mark A. Lever
Biogeosciences, 16, 3725–3746,
Maria Andrianaki, Juna Shrestha, Florian Kobierska, Nikolaos P. Nikolaidis, and Stefano M. Bernasconi
Hydrol. Earth Syst. Sci., 23, 3219–3232,Short summary
We tested the performance of the SWAT hydrological model after being transferred from a small Alpine watershed to a greater area. We found that the performance of the model for the greater catchment was satisfactory and the climate change simulations gave insights into the impact of climate change on our site. Assessment tests are important in identifying the strengths and weaknesses of the models when they are applied under extreme conditions different to the ones that were calibrated.
Maximilian Rieder, Wencke Wegner, Monika Horschinegg, Stefanie Klackl, Nereo Preto, Anna Breda, Susanne Gier, Urs Klötzli, Stefano M. Bernasconi, Gernot Arp, and Patrick Meister
Solid Earth, 10, 1243–1267,Short summary
The formation of dolomite (CaMg(CO3)2), an abundant mineral in Earth's geological record, is still incompletely understood. We studied dolomites embedded in a 100 m thick succession of coastal alluvial clays of Triassic age in the southern Alps. Observation by light microscopy and Sr isotopes suggests that dolomites may spontaneously from concentrated evaporating seawater, in coastal ephemeral lakes or tidal flats along the western margin of the Triassic Tethys sea.
Julian Helfenstein, Jannes Jegminat, Timothy I. McLaren, and Emmanuel Frossard
Biogeosciences, 15, 105–114,Short summary
Soil solution phosphorus (P) turnover is essential for describing the bioavailability of this important nutrient. Here, we provide a derivation of calculating soil solution P turnover using parameters obtained from isotope exchange kinetic experiments. We then calculated and analyzed soil solution P turnover for 217 soils and for 18 long-term P fertilizer field experiments worldwide. Our study thus provides important insights on P dynamics in soils.
Emmanuel Frossard, Nina Buchmann, Else K. Bünemann, Delwende I. Kiba, François Lompo, Astrid Oberson, Federica Tamburini, and Ouakoltio Y. A. Traoré
SOIL, 2, 83–99,
F. Kobierska, T. Jonas, J. W. Kirchner, and S. M. Bernasconi
Hydrol. Earth Syst. Sci., 19, 3681–3693,
Related subject area
Biogeochemistry: Stable Isotopes & Other TracersModern silicon dynamics of a small high-latitude subarctic lakeRadium-228-derived ocean mixing and trace element inputs in the South AtlanticNitrogen isotopic fractionations during nitric oxide production in an agricultural soilSilicon uptake and isotope fractionation dynamics by crop speciesBarium stable isotopes as a fingerprint of biological cycling in the Amazon River basinBottomland hardwood forest growth and stress response to hydroclimatic variation: evidence from dendrochronology and tree ring Δ13C valuesN2O isotope approaches for source partitioning of N2O production and estimation of N2O reduction – validation with the 15N gas-flux method in laboratory and field studiesTechnical note: Single-shell δ11B analysis of Cibicidoides wuellerstorfi using femtosecond laser ablation MC-ICPMS and secondary ion mass spectrometrySources and cycling of nitrogen in a New England river discerned from nitrate isotope ratiosBiogeochemical evidence of anaerobic methane oxidation and anaerobic ammonium oxidation in a stratified lake using stable isotopesEvaluating the response of ẟ13C in Haloxylon ammodendron, a dominant C4 species in Asian desert ecosystem, to water and nitrogen addition as well as the availability of its ẟ13C as the indicator of water use-efficiencyEffects of 238U variability and physical transport on water column 234Th downward fluxes in the coastal upwelling system off PeruDo degree and rate of silicate weathering depend on plant productivity?Alpine Holocene tree-ring dataset: age-related trends in the stable isotopes of cellulose show species-specific patternsIdeas and perspectives: The same carbon behaves like different elements – an insight into position-specific isotope distributionsSeasonal dynamics of the COS and CO2 exchange of a managed temperate grasslandLeaf-scale quantification of the effect of photosynthetic gas exchange on Δ17O of atmospheric CO2The stable carbon isotope signature of methane produced by saprotrophic fungiUnderstanding the effects of early degradation on isotopic tracers: implications for sediment source attribution using compound-specific isotope analysis (CSIA)Oxygen isotope composition of waters recorded in carbonates in strong clumped and oxygen isotopic disequilibriumIsotopic evidence for alteration of nitrous oxide emissions and producing pathways' contribution under nitrifying conditionsTrace element composition of size-fractionated suspended particulate matter samples from the Qatari Exclusive Economic Zone of the Arabian Gulf: the role of atmospheric dustBenthic carbon fixation and cycling in diffuse hydrothermal and background sediments in the Bransfield Strait, AntarcticaChanges in gross oxygen production, net oxygen production, and air-water gas exchange during seasonal ice melt in Whycocomagh Bay, a Canadian estuary in the Bras d'Or Lake systemHigh resolution 14C bomb-peak dating and climate response analyses of subseasonal stable isotope signals in wood of the African baobab – A case study from OmanPlants or bacteria? 130 years of mixed imprints in Lake Baldegg sediments (Switzerland), as revealed by compound-specific isotope analysis (CSIA) and biomarker analysisCommercial traceability of Arapaima spp. fisheries in the Amazon basin: can biogeochemical tags be useful?Distribution of Fe isotopes in particles and colloids in the salinity gradient along the Lena River plume, Laptev SeaEarly season N2O emissions under variable water management in rice systems: source-partitioning emissions using isotope ratios along a depth profileEvolution of 231Pa and 230Th in overflow waters of the North AtlanticSouthern Ocean controls of the vertical marine δ13C gradient – a modelling studyNegligible isotopic fractionation of nitrogen within temperate Zostera spp. meadowsEcosystem fluxes of carbonyl sulfide in an old-growth forest: temporal dynamics and responses to diffuse radiation and heat wavesExport flux of unprocessed atmospheric nitrate from temperate forested catchments: a possible new index for nitrogen saturationSilicon isotopes of deep sea sponges: new insights into biomineralisation and skeletal structureLatitudinal trends in stable isotope signatures and carbon-concentrating mechanisms of northeast Atlantic rhodolithsSalinity control on Na incorporation into calcite tests of the planktonic foraminifera Trilobatus sacculifer – evidence from culture experiments and surface sedimentsThe silicon stable isotope distribution along the GEOVIDE section (GEOTRACES GA-01) of the North Atlantic OceanTracing water masses with 129I and 236U in the subpolar North Atlantic along the GEOTRACES GA01 sectionEnvironmental and taxonomic controls of carbon and oxygen stable isotope composition in Sphagnum across broad climatic and geographic rangesEstimation of isotope variation of N2O during denitrification by Pseudomonas aureofaciens and Pseudomonas chlororaphis: implications for N2O source apportionmentImmediate increase in isotopic enrichment in small mammals following the expansion of a great cormorant colonyStomatal control of leaf fluxes of carbonyl sulfide and CO2 in a Typha freshwater marshThe triple oxygen isotope composition of phytoliths as a proxy of continental atmospheric humidity: insights from climate chamber and climate transect calibrationsShort-term fate of intertidal microphytobenthos carbon under enhanced nutrient availability: a 13C pulse-chase experimentδ11B as monitor of calcification site pH in divergent marine calcifying organismsCatchment tracers reveal discharge, recharge and sources of groundwater-borne pollutants in a novel lake modelling approachStable isotopic constraints on global soil organic carbon turnoverSpatial variations in snowpack chemistry, isotopic composition of NO3− and nitrogen deposition from the ice sheet margin to the coast of western GreenlandGlacial–interglacial changes and Holocene variations in Arabian Sea denitrification
Petra Zahajská, Carolina Olid, Johanna Stadmark, Sherilyn C. Fritz, Sophie Opfergelt, and Daniel J. Conley
Biogeosciences, 18, 2325–2345,Short summary
The drivers of high accumulation of single-cell siliceous algae (diatoms) in a high-latitude lake have not been fully characterized before. We studied silicon cycling of the lake through water, radon, silicon, and stable silicon isotope balances. Results showed that groundwater brings 3 times more water and dissolved silica than the stream inlet. We demonstrate that groundwater discharge and low sediment deposition have driven the high diatom accumulation in the studied lake in the past century.
Yu-Te Hsieh, Walter Geibert, E. Malcolm S. Woodward, Neil J. Wyatt, Maeve C. Lohan, Eric P. Achterberg, and Gideon M. Henderson
Biogeosciences, 18, 1645–1671,Short summary
The South Atlantic near 40° S is one of the high-productivity and most dynamic nutrient regions in the oceans, but the sources and fluxes of trace elements (TEs) to this region remain unclear. This study investigates seawater Ra-228 and provides important constraints on ocean mixing and dissolved TE fluxes to this region. Vertical mixing is a more important source than aeolian or shelf inputs in this region, but particulate or winter deep-mixing inputs may be required to balance the TE budgets.
Zhongjie Yu and Emily M. Elliott
Biogeosciences, 18, 805–829,Short summary
In this study, we demonstrated distinct nitrogen isotope effects for nitric oxide (NO) production from major microbial and chemical NO sources in an agricultural soil. These results highlight characteristic bond-forming and breaking mechanisms associated with microbial and chemical NO production and implicate that simultaneous isotopic analyses of NO and nitrous oxide (N2O) can lead to unprecedented insights into the sources and processes controlling NO and N2O emissions from agricultural soils.
Daniel A. Frick, Rainer Remus, Michael Sommer, Jürgen Augustin, Danuta Kaczorek, and Friedhelm von Blanckenburg
Biogeosciences, 17, 6475–6490,Short summary
Silicon is taken up by some plants to increase structural stability and to develop stress resistance and is rejected by others. To explore the underlying mechanisms, we used the stable isotopes of silicon that shift in their relative abundance depending on the biochemical transformation involved. On species with a rejective (tomato, mustard) and active (wheat) uptake mechanism, grown in hydroculture, we found that the transport of silicic acid is controlled by the precipitation of biogenic opal.
Quentin Charbonnier, Julien Bouchez, Jérôme Gaillardet, and Éric Gayer
Biogeosciences, 17, 5989–6015,Short summary
The abundance and isotope composition of the trace metal barium (Ba) allows us to track and quantify nutrient cycling throughout the Amazon Basin. In particular, we show that the Ba biological fingerprint evolves from that of a strong net nutrient uptake in the mountainous area of the Andes towards efficient nutrient recycling on the plains of the Lower Amazon. Our study highlights the fact that the geochemical signature of rock-derived nutrients transported by the Amazon is scarred by life.
Ajinkya G. Deshpande, Thomas W. Boutton, Ayumi Hyodo, Charles W. Lafon, and Georgianne W. Moore
Biogeosciences, 17, 5639–5653,Short summary
Wetland forests in the southern USA are threatened by changing climate and human-induced pressures. We used tree ring widths and C isotopes as indicators of forest growth and physiological stress, respectively, and compared these to past climate data. We observed that vegetation growing in the drier patches is susceptible to stress, while vegetation growth and physiology in wetter patches is less sensitive to unfavorable environmental conditions, highlighting the importance of optimal wetness.
Dominika Lewicka-Szczebak, Maciej Piotr Lewicki, and Reinhard Well
Biogeosciences, 17, 5513–5537,Short summary
We present the first validation of N2O isotopic approaches for estimating N2O source pathways and N2O reduction. These approaches are widely used for tracing soil nitrogen cycling, but the results of these estimations are very uncertain. Here we report the results from parallel treatments allowing for precise validation of these approaches, and we propose the best strategies for results interpretation, including the new idea of an isotope model integrating three isotopic signatures of N2O.
Markus Raitzsch, Claire Rollion-Bard, Ingo Horn, Grit Steinhoefel, Albert Benthien, Klaus-Uwe Richter, Matthieu Buisson, Pascale Louvat, and Jelle Bijma
Biogeosciences, 17, 5365–5375,Short summary
The isotopic composition of boron in carbonate shells of marine unicellular organisms is a popular tool to estimate seawater pH. Usually, many shells need to be dissolved and measured for boron isotopes, but the information on their spatial distribution is lost. Here, we investigate two techniques that allow for measuring boron isotopes within single shells and show that they yield robust mean values but provide additional information on the heterogeneity within and between single shells.
Veronica R. Rollinson, Julie Granger, Sydney C. Clark, Mackenzie L. Blanusa, Claudia P. Koerting, Jamie M. P. Vaudrey, Lija A. Treibergs, Holly C. Westbrook, Catherine M. Matassa, Meredith K. Hastings, and Craig R. Tobias
Revised manuscript accepted for BGShort summary
We measured nutrients and the naturally occurring nitrogen (N) and oxygen (O) stable isotope ratios of nitrate discharged from a New England river over an annual cycle, to monitor N loading and identify dominant sources from the watershed. We uncovered a seasonality to loading and sources of N from the watershed. Seasonality in the nitrate isotope ratios also informed on N cycling, conforming to theoretical expectations of riverine nutrient cycling.
Florian Einsiedl, Anja Wunderlich, Mathieu Sebilo, Ömer K. Coskun, William D. Orsi, and Bernhard Mayer
Biogeosciences, 17, 5149–5161,Short summary
Nitrate pollution of freshwaters and methane emissions into the atmosphere are crucial factors in deteriorating the quality of drinking water and in contributing to global climate change. Here, we report vertical concentration and stable isotope profiles of CH4, NO3-, NO2-, and NH4+ in the water column of Fohnsee (southern Bavaria, Germany) that may indicate linkages between nitrate-dependent anaerobic methane oxidation and the anaerobic oxidation of ammonium.
Zixun Chen, Xuejun Liu, Xiaoqing Cui, Yaowen Han, Guoan Wang, and Jiazhu Li
Revised manuscript accepted for BGShort summary
ẟ13C in plants is a sensitive long-term indicator of physiological acclimatization. The present study suggests that precipitation change and increasing atmospheric N deposition have little impact on ẟ13C of H. ammodendron, a dominant plant in Central Asia deserts, but affect its gas exchange. In addition, this study shows that ẟ13C of H. ammodendron could not indicate its water use efficiency (WUE), suggesting that whether ẟ13C of C4 plants indicates WUE is species-specific dependent.
Ruifang C. Xie, Frédéric A. C. Le Moigne, Insa Rapp, Jan Lüdke, Beat Gasser, Marcus Dengler, Volker Liebetrau, and Eric P. Achterberg
Biogeosciences, 17, 4919–4936,Short summary
Thorium-234 (234Th) is widely used to study carbon fluxes from the surface ocean to depth. But few studies stress the relevance of oceanic advection and diffusion on the downward 234Th fluxes in nearshore environments. Our study in offshore Peru showed strong temporal variations in both the importance of physical processes on 234Th flux estimates and the oceanic residence time of 234Th, whereas salinity-derived seawater 238U activities accounted for up to 40 % errors in 234Th flux estimates.
Ralf A. Oeser and Friedhelm von Blanckenburg
Biogeosciences, 17, 4883–4917,Short summary
We present a novel strategy to decipher the relative impact of biogenic and abiotic drivers of weathering. We parameterized the nutrient fluxes in four ecosystems along a climate and vegetation gradient situated on the Chilean Coastal Cordillera. We investigated how nutrient demand by plants drives weathering. We found that the increase in biomass nutrient demand is accommodated by faster nutrient recycling rather than an increase in the weathering–release rates.
Tito Arosio, Malin M. Ziehmer, Kurt Nicolussi, Christian Schlüchter, and Markus Leuenberger
Biogeosciences, 17, 4871–4882,Short summary
Stable isotopes in tree-ring cellulose are tools for climatic reconstructions, but interpretation is challenging due to nonclimate trends. We analyzed the tree-age trends in tree-ring isotopes of deciduous larch and evergreen cembran pine. Samples covering the whole Holocene were collected at the tree line in the Alps. For cambial ages over 100 years, we prove the absence of age trends in δD, δ18O, and δ13C for both species. For lower cambial ages, trends differ for each isotope and species.
Yuyang He, Xiaobin Cao, and Huiming Bao
Biogeosciences, 17, 4785–4795,Short summary
Different carbon sites in a large organic molecule have different isotope compositions. Different carbon sites may not have the chance to exchange isotopes at all. The lack of appreciation of this notion might be blamed for an unsettled debate on the thermodynamic state of an organism. Here we demonstrate using minerals, N2O, and acetic acid that the dearth of exchange among different carbon sites renders them as independent as if they were different elements in organic molecules.
Felix M. Spielmann, Albin Hammerle, Florian Kitz, Katharina Gerdel, and Georg Wohlfahrt
Biogeosciences, 17, 4281–4295,Short summary
Carbonyl sulfide (COS) can be used as a proxy for plant photosynthesis on an ecosystem scale. However, the relationships between COS and CO2 fluxes and their dependence on daily to seasonal changes in environmental drivers are still poorly understood. We examined COS and CO2 ecosystem fluxes above an agriculturally used mountain grassland for 6 months. Harvesting of the grassland disturbed the otherwise stable COS-to-CO2 uptake ratio. We even found the canopy to release COS during those times.
Getachew Agmuas Adnew, Thijs L. Pons, Gerbrand Koren, Wouter Peters, and Thomas Röckmann
Biogeosciences, 17, 3903–3922,Short summary
We measured the effect of photosynthesis, the largest flux in the carbon cycle, on the triple oxygen isotope composition of atmospheric CO2 at the leaf level during gas exchange using three plant species. The main factors that limit the impact of land vegetation on the triple oxygen isotope composition of atmospheric CO2 are identified, characterized and discussed. The effect of photosynthesis on the isotopic composition of CO2 is commonly quantified as discrimination (ΔA).
Moritz Schroll, Frank Keppler, Markus Greule, Christian Eckhardt, Holger Zorn, and Katharina Lenhart
Biogeosciences, 17, 3891–3901,Short summary
Fungi have recently been identified to produce the greenhouse gas methane. Here, we investigated the stable carbon isotope values of methane produced by saprotrophic fungi. Our results show that stable isotope values of methane from fungi are dependent on the fungal species and the metabolized substrate. They cover a broad range and overlap with stable carbon isotope values of methane reported for methanogenic archaea, the thermogenic degradation of organic matter, and other eukaryotes.
Pranav Hirave, Guido L. B. Wiesenberg, Axel Birkholz, and Christine Alewell
Biogeosciences, 17, 2169–2180,Short summary
Sediment input into water bodies is a prominent threat to freshwater ecosystems. We tested the stability of tracers employed in freshwater sediment tracing based on compound-specific isotope analysis during early degradation in soil. While bulk δ13C values showed no stability, δ13C values of plant-derived fatty acids and n-alkanes were stably transferred to the soil without soil particle size dependency after an early degradation in organic horizons, thus indicating their suitability as tracers.
Caroline Thaler, Amandine Katz, Magali Bonifacie, Bénédicte Ménez, and Magali Ader
Biogeosciences, 17, 1731–1744,Short summary
Paleoenvironment reconstructions, retrieved from δ18O and Δ47 values measured in carbonate, are compromised when crystallization occurs in isotopic disequilibrium. We show that some paleoenvironmental information can still be retrieved from these paired disequilibrium Δ47 and δ18O values. The possibility of retrieving information on paleowaters, sediments' interstitial waters, or organisms' body water at the carbonate precipitation loci will help understand past Earth and life evolution.
Guillaume Humbert, Mathieu Sébilo, Justine Fiat, Longqi Lang, Ahlem Filali, Véronique Vaury, Mathieu Spérandio, and Anniet M. Laverman
Biogeosciences, 17, 979–993,Short summary
Mitigating emissions of the greenhouse gas N2O requires understanding of the relative contribution of its producing processes in response to environmental variables. We show, using isotopic analysis, that N2O emissions from a nitrifying system were sensitive to oxygenation, temperature and NH4+ concentrations with nitrite reduction being the main N2O source. Temperature appears to be the main control on N2O production, due to its dissimilar effects on ammonium and nitrite oxidizing activities.
Oguz Yigiterhan, Ebrahim Mohd Al-Ansari, Alex Nelson, Mohamed Alaa Abdel-Moati, Jesse Turner, Hamood Abdulla Alsaadi, Barbara Paul, Ibrahim Abdullatif Al-Maslamani, Mehsin Abdulla Al-Ansi Al-Yafei, and James W. Murray
Biogeosciences, 17, 381–404,Short summary
We analyze net-tow samples of plankton and associated particulate matter from the Exclusive Economic Zone, Qatar, Arabian Gulf, using net tows with mesh sizes of 50 and 200 μm to examine the composition of plankton populations. We also focus on the role and composition of the atmospheric dust, representative of terrigenous material, deposited in the Gulf. We concluded that Al, Fe, Cr, Co, Mn, Ni, Pb, and Li are of dust origin and As, Cd, Cu, Mo, Zn, and Ca are of anthropogenic/biogenic origin.
Clare Woulds, James B. Bell, Adrian G. Glover, Steven Bouillon, and Louise S. Brown
Biogeosciences, 17, 1–12,Short summary
Sedimented hydrothermal vents occur where heated, mineral-rich (hydrothermal) water seeps through seafloor sediments. They host chemosynthetic microbes, which use chemical energy to fix dissolved carbon dioxide into sugars (chemosynthesis). We conducted carbon tracing experiments, and observed chemosynthesis at both vent and non-vent sites. Thus, chemosynthesis occurred over a much larger area than expected, suggesting it is more widespread than previously thought.
Cara C. Manning, Rachel H. R. Stanley, David P. Nicholson, Brice Loose, Ann Lovely, Peter Schlosser, and Bruce G. Hatcher
Biogeosciences, 16, 3351–3376,Short summary
We measured rates of biological activity and gas exchange in a Canadian estuary during ice melt. We quantified gas exchange using inert, deliberately released tracers and found that the gas transfer rate at > 90 % ice cover was 6 % of the rate for nearly ice-free conditions. We measured oxygen concentration and isotopic composition and used the data to detect changes in the rates of photosynthesis and respiration (autotrophy and heterotrophy) as the ice melted.
Franziska Slotta, Lukas Wacker, Frank Riedel, Karl-Uwe Heußner, Kai Hartmann, and Gerhard Helle
Revised manuscript accepted for BGShort summary
The African baobab is a challenging climate- and environmental archive for its semi-arid habitat due to dating uncertainties and parenchyma-rich wood anatomy. Annually resolved F14C data of tree-ring cellulose (1941–2005) from a tree in Oman shows the annual character of the baobab's growth rings but were found up to 8.8 % lower than expected for 1964–1967. Sub-seasonal δ13C and δ18O patterns reveal years with low average monsoon rain as well as heavy rainfall events from pre-monsoonal cyclones.
Marlène Lavrieux, Axel Birkholz, Katrin Meusburger, Guido L. B. Wiesenberg, Adrian Gilli, Christian Stamm, and Christine Alewell
Biogeosciences, 16, 2131–2146,Short summary
A fingerprinting approach using compound-specific stable isotopes was applied to a lake sediment core to reconstruct erosion processes over the past 150 years in a Swiss catchment. Even though the reconstruction of land use and eutrophication history was successful, the observation of comparatively low δ13C values of plant-derived fatty acids in the sediment suggests their alteration within the lake. Thus, their use as a tool for source attribution in sediment cores needs further investigation.
Luciana A. Pereira, Roberto V. Santos, Marília Hauser, Fabrice Duponchelle, Fernando Carvajal, Christophe Pecheyran, Sylvain Bérail, and Marc Pouilly
Biogeosciences, 16, 1781–1797,Short summary
This study presents the first step for a chemical origin certification of pirarucu fishery in the Amazon. A preliminary isotopic tool to improve the actual tracking system integrates ecological, social, and economic aspects of Amazon dynamics. The geographic origin validation of farmed and wild fishes contributes to environmental and social practices, secures food and income to communities, helps manage endangered species, reinforces aquaculture, and combats illegal fisheries.
Sarah Conrad, Johan Ingri, Johan Gelting, Fredrik Nordblad, Emma Engström, Ilia Rodushkin, Per S. Andersson, Don Porcelli, Örjan Gustafsson, Igor Semiletov, and Björn Öhlander
Biogeosciences, 16, 1305–1319,Short summary
Iron analysis of the particulate, colloidal, and truly dissolved fractions along the Lena River freshwater plume showed that the particulate iron dominates close to the coast. Over 99 % particulate and about 90 % colloidal iron were lost, while the truly dissolved phase was almost constant. Iron isotopes suggest that the shelf acts as a sink for particles and colloids with negative iron isotope values, while colloids with positive iron isotope values travel further out into the Arctic Ocean.
Elizabeth Verhoeven, Matti Barthel, Longfei Yu, Luisella Celi, Daniel Said-Pullicino, Steven Sleutel, Dominika Lewicka-Szczebak, Johan Six, and Charlotte Decock
Biogeosciences, 16, 383–408,Short summary
This study utilized state-of-the-art measurements of nitrogen isotopes to evaluate nitrogen cycling and to assess the biological sources of the potent greenhouse gas, N2O, in response to water-saving practices in rice systems. Water-saving practices did emit more N2O, and high N2O production had a lower 15N isotope signature. Modeling and visual interpretation indicate that these emissions mostly came from denitrification or nitrifier denitrification, controlled upstream by nitrification rates.
Feifei Deng, Gideon M. Henderson, Maxi Castrillejo, Fiz F. Perez, and Reiner Steinfeldt
Biogeosciences, 15, 7299–7313,Short summary
To better use Pa / Th to reconstruct deep water ventilation rate, we assessed controls on 230Th and 231Pa in the northern North Atlantic. With extended optimum multi-parameter analysis and CFC-based water-mass age, we found the imprint of young overflow water on Th and Pa and enhanced scavenging near the seafloor. A significantly higher advective loss of Pa to the south relative to Th in the Atlantic was estimated, supporting the use of Pa / Th for assessing basin-scale meridional transport.
Anne L. Morée, Jörg Schwinger, and Christoph Heinze
Biogeosciences, 15, 7205–7223,Short summary
Changes in the distribution of the carbon isotope 13C can be used to study the climate system if the governing processes (ocean circulation and biogeochemistry) are understood. We show the Southern Ocean importance for the global 13C distribution and that changes in 13C can be strongly influenced by biogeochemistry. Interpretation of 13C as a proxy for climate signals needs to take into account the effects of changes in biogeochemistry in addition to changes in ocean circulation.
Douglas G. Russell, Wei Wen Wong, and Perran L. M. Cook
Biogeosciences, 15, 7225–7234,Short summary
Nitrogen is an important nutrient in marine environments and is continually converted from one form to another. One way these processes can be investigated is by looking at the ratio of the 15N and 14N stable isotopes of different nitrogen-containing compounds. To date few studies have compared these ratios in seagrass beds, their associated sediments and the porewater NH4+ pool. The strong relationship between these nitrogen pools suggests that nitrogen is tightly recycled within seagrass beds.
Bharat Rastogi, Max Berkelhammer, Sonia Wharton, Mary E. Whelan, Frederick C. Meinzer, David Noone, and Christopher J. Still
Biogeosciences, 15, 7127–7139,Short summary
Carbonyl sulfide (OCS) has gained prominence as an independent tracer for gross primary productivity, which is usually modelled by partitioning net CO2 fluxes. Here, we present a simple empirical model for estimating ecosystem-scale OCS fluxes for a temperate old-growth forest and find that OCS sink strength scales with independently estimated CO2 uptake and is sensitive to the the fraction of downwelling diffuse light. We also examine the response of OCS and CO2 fluxes to sequential heat waves.
Fumiko Nakagawa, Urumu Tsunogai, Yusuke Obata, Kenta Ando, Naoyuki Yamashita, Tatsuyoshi Saito, Shigeki Uchiyama, Masayuki Morohashi, and Hiroyuki Sase
Biogeosciences, 15, 7025–7042,Short summary
To clarify the biological processing of nitrate within temperate forested catchments using unprocessed atmospheric nitrate exported from each catchment as a tracer, we continuously monitored stream nitrate concentrations and stable isotopic compositions in three forested catchments for more than 2 years. We concluded that the export flux of unprocessed atmospheric nitrate relative to the deposition flux in each forest ecosystem is applicable as an index for nitrogen saturation.
Lucie Cassarino, Christopher D. Coath, Joana R. Xavier, and Katharine R. Hendry
Biogeosciences, 15, 6959–6977,Short summary
Using a simple model, we show that the silicon isotopic composition of sponges can be used to estimate the silicic acid concentration of seawater, a key parameter linked to nutrient and carbon cycling. However, our data illustrate that skeletal type and growth rate also control silicon isotopic composition of sponges. Our study demonstrates the paleoceanographic utility of sponges as an archive for ocean silica content provided that suitable skeleton types are selected.
Laurie C. Hofmann and Svenja Heesch
Biogeosciences, 15, 6139–6149,Short summary
The ability of marine macroalgae to adapt to changing ocean chemistry will depend on the flexibility of their inorganic carbon uptake mechanisms across biogeographic ranges. Therefore, we investigated the plasticity of inorganic carbon uptake mechanisms in north Atlantic rhodoliths – free-living calcifying red algae that form important benthic habitats in coastal environments. We observed flexible mechanisms related to seawater DIC concentrations, indicating the potential for adaptation.
Jacqueline Bertlich, Dirk Nürnberg, Ed C. Hathorne, Lennart J. de Nooijer, Eveline M. Mezger, Markus Kienast, Steffanie Nordhausen, Gert-Jan Reichart, Joachim Schönfeld, and Jelle Bijma
Biogeosciences, 15, 5991–6018,
Jill N. Sutton, Gregory F. de Souza, Maribel I. García-Ibáñez, and Christina L. De La Rocha
Biogeosciences, 15, 5663–5676,Short summary
The silicon stable isotope distribution determined from samples collected from the North Atlantic Ocean indicates that water mass subduction and circulation are the dominant processes controlling the distribution of dissolved silicon in this region. In addition, these data provide a clear view of the direct interaction between northern and southern water masses and the extent to which the silicon isotope composition of these silica-poor waters is influenced by hydrography.
Maxi Castrillejo, Núria Casacuberta, Marcus Christl, Christof Vockenhuber, Hans-Arno Synal, Maribel I. García-Ibáñez, Pascale Lherminier, Géraldine Sarthou, Jordi Garcia-Orellana, and Pere Masqué
Biogeosciences, 15, 5545–5564,Short summary
The investigation of water mass transport pathways and timescales is important to understand the global ocean circulation. Following earlier studies, we use artificial radionuclides introduced to the oceans in the 1950s to investigate the water transport in the subpolar North Atlantic (SPNA). For the first time, we combine measurements of the long-lived iodine-129 and uranium-236 to confirm earlier findings/hypotheses and to better understand shallow and deep ventilation processes in the SPNA.
Gustaf Granath, Håkan Rydin, Jennifer L. Baltzer, Fia Bengtsson, Nicholas Boncek, Luca Bragazza, Zhao-Jun Bu, Simon J. M. Caporn, Ellen Dorrepaal, Olga Galanina, Mariusz Gałka, Anna Ganeva, David P. Gillikin, Irina Goia, Nadezhda Goncharova, Michal Hájek, Akira Haraguchi, Lorna I. Harris, Elyn Humphreys, Martin Jiroušek, Katarzyna Kajukało, Edgar Karofeld, Natalia G. Koronatova, Natalia P. Kosykh, Mariusz Lamentowicz, Elena Lapshina, Juul Limpens, Maiju Linkosalmi, Jin-Ze Ma, Marguerite Mauritz, Tariq M. Munir, Susan M. Natali, Rayna Natcheva, Maria Noskova, Richard J. Payne, Kyle Pilkington, Sean Robinson, Bjorn J. M. Robroek, Line Rochefort, David Singer, Hans K. Stenøien, Eeva-Stiina Tuittila, Kai Vellak, Anouk Verheyden, James Michael Waddington, and Steven K. Rice
Biogeosciences, 15, 5189–5202,Short summary
Peat constitutes a long-term archive for climate reconstruction by using the isotopic composition of carbon and oxygen. We analysed isotopes in two peat moss species across North America and Eurasia. Peat (moss tissue) isotope composition was predicted by soil moisture and isotopic composition of the rainwater but differed between species. Our results suggest that isotope composition can be used on a large scale for climatic reconstructions but that such models should be species-specific.
Joshua A. Haslun, Nathaniel E. Ostrom, Eric L. Hegg, and Peggy H. Ostrom
Biogeosciences, 15, 3873–3882,Short summary
N2O δ15N and δ18O values changed non-linearly during in vitro N2O production by bacterial denitrification of NO3−, an argument against their use in N2O apportionment. We present a novel approach for describing non-linear isotopic behaviour, which may be applied to other multi-step reactions. We also show that the intramolecular distribution of 15N in N2O, used to apportion N2O emissions to denitrification and nitrification, is unaffected by electron donor source and electron donor concentration.
Linas Balčiauskas, Raminta Skipitytė, Marius Jasiulionis, Laima Balčiauskienė, and Vidmantas Remeikis
Biogeosciences, 15, 3883–3891,Short summary
Effects of an expanding great cormorant colony on small mammals were evaluated. An increase of the number of bird pairs led to decreased δ13C and increased δ15N values in mice hair after the first year of nest appearance. Differences in isotopic signatures were related to species of rodents, pointing to the differences in their diet. Cormorant influence was indirect, the result of biological pollution from guano on rodent foods. Scaring cormorants from the colonies may have the opposite effect.
Wu Sun, Kadmiel Maseyk, Céline Lett, and Ulli Seibt
Biogeosciences, 15, 3277–3291,Short summary
Carbonyl sulfide (COS) is an emerging tracer to probe land photosynthesis at canopy to global scales, but the relationship between leaf COS and CO2 fluxes needed for this application is poorly quantified. With in situ leaf fluxes of COS and CO2 measured in a freshwater marsh, we show that light and vapor deficit control the relationship between leaf COS and CO2 fluxes by regulating stomatal conductance. Our findings support the use of COS as a tracer for canopy photosynthesis.
Anne Alexandre, Amarelle Landais, Christine Vallet-Coulomb, Clément Piel, Sébastien Devidal, Sandrine Pauchet, Corinne Sonzogni, Martine Couapel, Marine Pasturel, Pauline Cornuault, Jingming Xin, Jean-Charles Mazur, Frédéric Prié, Ilhem Bentaleb, Elizabeth Webb, Françoise Chalié, and Jacques Roy
Biogeosciences, 15, 3223–3241,Short summary
There is a lack of proxies suitable for reconstructing, in a quantitative way, past changes in continental atmospheric humidity, which is a key climate parameter. Here, we demonstrate through climate chamber and climate transect calibrations that the triple oxygen isotope composition of phytoliths offers a potential for reconstructing changes in relative humidity.
Philip M. Riekenberg, Joanne M. Oakes, and Bradley D. Eyre
Biogeosciences, 15, 2873–2889,Short summary
Shallow coastal waters are increasingly experiencing increased nutrient loading. Sediment algae within these systems are responsible for a large portion of C production, but we have limited knowledge of what happens to sediment microbial processing of MPB-C under increased nutrient conditions. This work examines how C-processing pathways change after increased short-term nutrient exposure, finding shifts in processing between microbial groups and increased export of algal C from the sediment.
Jill N. Sutton, Yi-Wei Liu, Justin B. Ries, Maxence Guillermic, Emmanuel Ponzevera, and Robert A. Eagle
Biogeosciences, 15, 1447–1467,Short summary
The boron isotope composition of marine biogenic carbonates has been studied as a proxy for monitoring past changes in seawater pH and carbonate chemistry. We highlight the potential utility of the boron isotope composition of marine biogenic carbonates as a proxy of calcifying site pH for a wide range of calcifying taxa and the importance of using species-specific seawater-pH–boron isotope calibrations when reconstructing seawater pH from the boron isotope composition of biogenic carbonates.
Emil Kristensen, Mikkel Madsen-Østerbye, Philippe Massicotte, Ole Pedersen, Stiig Markager, and Theis Kragh
Biogeosciences, 15, 1203–1216,Short summary
We propose a novel modelling approach enabling swift hydrological surveys based on multiple conservative and non-conservative tracers to estimate water retention time, groundwater discharge sites, fractions of water originating from the discharge sites, groundwater recharge sites and sites that are especially important in regard to groundwater recharge. Thus we provide a whole lake hydrological survey while pinpointing sources of pollutants like colored dissolved organic matter and nutrients.
Chao Wang, Benjamin Z. Houlton, Dongwei Liu, Jianfeng Hou, Weixin Cheng, and Edith Bai
Biogeosciences, 15, 987–995,Short summary
Soil contains a large amount of organic carbon and plays a crucial role in regulating Earth's C cycle and climate system. In this study, we collected soil-carbon isotope data within a 1 m depth globally and provided an isotope-based approach for understanding soil carbon decomposition rate. Compared with other methods, utilization of C isotope composition ratios in the soil profile provides an independent approach that does not rely on disruption of plant-soil-microbe interactions.
Chris J. Curtis, Jan Kaiser, Alina Marca, N. John Anderson, Gavin Simpson, Vivienne Jones, and Erika Whiteford
Biogeosciences, 15, 529–550,Short summary
Few studies have investigated the atmospheric deposition of nitrate in the Arctic or its impacts on Arctic ecosystems. We collected late-season snowpack from three regions in western Greenland from the coast to the edge of the ice sheet. We found major differences in nitrate concentrations (lower at the coast) and deposition load (higher). Nitrate in snowpack undergoes losses and isotopic enrichment which are greatest in inland areas; hence deposition impacts may be greatest at the coast.
Birgit Gaye, Anna Böll, Joachim Segschneider, Nicole Burdanowitz, Kay-Christian Emeis, Venkitasubramani Ramaswamy, Niko Lahajnar, Andreas Lückge, and Tim Rixen
Biogeosciences, 15, 507–527,Short summary
The Arabian Sea has one of the most severe oxygen minima of the world's oceans between about 100 and 1200 m of water depth and is therefore a major oceanic nitrogen sink. Stable nitrogen isotopic ratios in sediments record changes in oxygen concentrations and were studied for the last 25 kyr. Oxygen concentrations dropped at the end of the last glacial and became further reduced during the Holocene, probably due to the increasing age of the low-oxygen water mass.
Anderson, G.: Assessing organic phosphorus in soils, in: The role of phosphorus in agriculture, Madison, USA, Amer. Soc. Agron., 411–432, 1980.
Angert, A., Weiner, T., Mazeh, S., Tamburini, F., Frossard, E., Bernasconi, S. M., and Sternberg, M.: Seasonal variability of soil phosphate stable oxygen isotopes in rainfall manipulation experiments, Geochim. Cosmochim. Acta, 75, 4216–4227, 2011.
Angert, A., Weiner, T., Mazeh, S., and Sternberg, M: Soil phosphate stable oxygen isotopes across rainfall and bedrock gradients, Environ. Sci. Technol., 46, 2156–2162, 2012.
Annaheim, K. E., Rufener, C. B., Frossard, E., and Bünemann, E. K.: Hydrolysis of organic phosphorus in soil water suspensions after addition of phosphatase enzymes, Biol. Fertil. Soil., 49, 1203–1213, 2013.
Blake, R. E., O'Neil, J. R., and Surkov, A. V.: Biogeochemical cycling of phosphorus: Insights from oxygen isotope effects of phosphoenzymes, Am. J. Sci., 305, 596–620, 2005.
Chang, S. J. and Blake, R. E.: Precise calibration of equilibrium oxygen isotope fractionations between dissolved phosphate and water from 3 to 37 °C, Geochim. Cosmochim. Acta, 150, 314–329, 2015.
Cohn, M.: Mechanisms of cleavage of glucose-1-phosphate, J. Biol. Chem., 180, 771–781, 1949.
Cohn, M.: Phosphate-water exchange reaction catalyzed by inorganic pyrophosphatase of yeast, J. Biol. Chem., 230, 369–380, 1958.
Cosgrove, D. J. and Irving, G. C. J.: Inositol phosphates: their chemistry, biochemistry, and physiology, Elsevier Scientific Pub. Co., Distributors for the US and Canada, Elsevier/North-Holland, Amsterdam, New York,New York, 1980.
Dalal, R. C.: Soil organic phosphorus, Adv. Agron., 29, 83–117, 1977.
Doolette, A. L., Smernik, R. J., and Dougherty, W. J.: Rapid decomposition of phytate applied to a calcareous soil demonstrated by a solution 31P NMR study, Eur. J. Soil Sci., 61, 563–575, 2010.
Dvořáková, J.: Phytase: Sources, preparation and exploitation, Folia Microbiol., 43, 323–338, 1998.
Ehlers, K., Bakken, L. R., Frostegård, A., Frossard, E., and Bünemann, E. K.: Phosphorus limitation in a Ferralsol: Impact on microbial activity and cell internal P pools, Soil Biol. Biochem., 42, 558–566, 2010.
George, T. S., Richardson, A. E., and Simpson, R. J.: Behaviour of plant-derived extracellular phytase upon addition to soil, Soil Biol. Biochem., 37, 977–988, 2005.
Gibson, D. M. and Ullah A., H. J.: Purification and characterization of phytase from cotyledons of germinating soybean seeds, Arch. Biochem. Biophys., 260, 503–513, 1988.
Gross, A. and Angert, A.: What processes control the oxygen isotopes of soil bio-available phosphate?, Geochim. Cosmochim. Acta, 159, 100–111, 2015.
Gross, A., Nishri, A., and Angert, A.: Use of phosphate oxygen isotopes for identifying atmospheric-P sources: A case study at Lake Kinneret, Environ. Sci. Technol., 47, 2721–2727, 2013.
Hayes, J. E., Richardson, A. E., and Simpson, R. J.: Phytase and acid phosphatase activities in extracts from roots of temperate pasture grass and legume seedlings, Functional Plant Biol., 26, 801–809, 1999.
Kim, E. E. and Wyckoff, H. W.: Reaction mechanism of alkaline phosphatase based on crystal structures: Two-metal ion catalysis, J. Mol. Biol., 218, 449–464, 1991.
Knöfel, T. and Sträter, N.: Mechanism of hydrolysis of phosphate esters by the dimetal center of 5´-nucleotidase based on crystal structures, J. Mol. Biol., 309, 239–254, 2001.
Kolodny, Y., Luz, B., and Navon, O.: Oxygen isotope variations in phosphate of biogenic apatites, I. Fish bone apatite–rechecking the rules of the game, Earth Planet. Sci. Lett., 64, 398–404, 1983a.
Kostrewa, D., Grüninger-Leitch, F., D'Arcy, A., Broger, C., Mitchell, D., and van Loon, A. P. G. M.: Crystal structure of phytase from Aspergillus ficuum at 2.5 Å resolution, Nat. Struct. Biol., 4, 185–190, 1997.
Kostrewa, D., Wyss, M., D'Arcy, A., and van Loon, A. P. G.: Crystal structure of Aspergillus niger pH 2.5 acid phosphatase at 2.4 Å resolution, J. Mol. Biol., 288, 965–974, 1999.
Li, J., Hegeman, C. E., Hanlon, R. W., Lacy, G. H., Denbow, D. M., and Grabau, E. A.: Secretion of active recombinant phytase from soybean cell-suspension cultures, Plant Physiol., 114, 1103–1111, 1997.
Li, M., Osaki, M., Rao, I. M., and Tadano, T.: Secretion of phytase from the roots of several plant species under phosphorus-deficient conditions, Plant Soil, 195, 161–169, 1997.
Liang, Y. and Blake, R.E.: Oxygen isotope signature of Pi regeneration from organic compounds by phosphomonoesterases and photooxidation, Geochim. Cosmochim. Acta, 70, 3957–3969, 2006.
Liang, Y. and Blake, R. E.: Compound- and enzyme-specific phosphodiester hydrolysis mechanisms revealed by δ18O of dissolved inorganic phosphate: Implications for marine P cycling, Geochim. Cosmochim. Acta, 73, 3782–3794, 2009.
Lim, D., Golovan, S., Forsberg, C. W., and Jia, Z.: Crystal structures of Escherichia coli phytase and its complex with phytate, Nat. Struct. Mol. Biol., 7, 108–113, 2000..
Lindqvist, Y., Schneider, G., and Vihko, P.: Crystal structures of rat acid phosphatase complexed with the transition-state analogs vanadate and molybdate, Eur. J. Biochem., 221, 139–142, 1994.
Longinelli, A. and Nuti, S.: Revised phosphate-water isotopic temperature scale, Earth Planet. Sci. Lett., 19, 373–376, 1973.
Lott, J. N. A., Ockenden, I., Raboy, V., and Batten, G. D.: Phytic acid and phosphorus in crop seeds and fruits: a global estimate, Seed Sci. Res., 10, 11–33, 2000.
Lung, S.-C. and Lim, B. L.: Assimilation of phytate-phosphorus by the extracellular phytase activity of tobacco (Nicotiana tabacum) is affected by the availability of soluble phytate, Plant Soil, 279, 187–199, 2006.
McKercher, R. B. and Anderson, G.: Organic phosphate sorption by neutral and basic soils, Commun. Soil Sci. Plant Anal., 20, 723–732, 1989.
McLaughlin, K., Paytan, A., Kendall, C., and Silva, S.: Oxygen isotopes of phosphatic compounds – Application for marine particulate matter, sediments and soils, Mar. Chem., 98, 148–155, 2006c.
Mullaney, E. J. and Ullah, A. H. J.: The term phytase comprises several different classes of enzymes, Biochem. Biophys. Res. Commun., 312, 179–184, 2003.
Ognalaga, M., Frossard, E., and Thomas, F.: Glucose-1-phosphate and Myo-inositol Hexaphosphate Adsorption Mechanisms on Goethite, Soil Sci. Soc. Am. J., 58, 332–337, 1994.
Oh, B.-C., Choi, W.-C., Park, S., Kim, Y., and Oh, T.-K.: Biochemical properties and substrate specificities of alkaline and histidine acid phytases, Appl. Microbiol. Biotechnol., 63, 362–372, 2004.
Ohno, T. and Zibilske, L. M.: Determination of Low Concentrations of Phosphorus in Soil Extracts Using Malachite Green, Soil Sci. Soc. Am. J., 55, 892 pp., 1991.
O'Neil, J. R., Vennemann, T. W., and McKenzie, W. F.: Effects of speciation on equilibrium fractionations and rates of oxygen isotope exchange between (PO4)aq and H2O, Geochim. Cosmochim. Acta, 67, 3135–3144, 2003.
Ortlund, E., LaCount, M. W., and Lebioda, L.: Crystal structures of human prostatic acid phosphatase in complex with a phosphate ion and α-Benzylaminobenzylphosphonic acid update the mechanistic picture and offer new insights into inhibitor design, Biochemical, 42, 383–389, 2003.
Paytan, A., Kolodny, Y., Neori, A., and Luz, B.: Rapid biologically mediated oxygen isotope exchange between water and phosphate, Global Biogeochem. Cy., 16, 13-1–13-8, 2002.
Pfahler, V., Dürr-Auster, T., Tamburini, F., M. Bernasconi, S., and Frossard, E.: 18O enrichment in phosphorus pools extracted from soybean leaves, New Phytol., 197, 186–193, 2013.
Raboy, V.: Accumulation and Storage of Phosphate and Minerals, in: Cellular and Molecular Biology of Plant Seed Development, edited by: Larkins, B. A. and Vasil, I. K., Advances in Cellular and Molecular Biology of Plants, Springer Netherlands, 441–477, 1997.
Richardson, A. E., Hadobas, P. A., and Hayes, J. E.: Acid phosphomonoesterase and phytase activities of wheat (Triticum aestivum L.) roots and utilization of organic phosphorus substrates by seedlings grown in sterile culture, Plant Cell Environ., 23, 397–405, 2000.
Richardson, A. E., Hadobas, P. A., and Hayes, J. E.: Extracellular secretion of Aspergillus phytase from Arabidopsis roots enables plants to obtain phosphorus from phytate, Plant J., 25, 641–649, 2001.
Richardson, A. E., Lynch, J. P., Ryan, P. R., Delhaize, E., Smith, F. A., Smith, S. E., Harvey, P. R., Ryan, M. H., Veneklaas, E. J., Lambers, H., Oberson, A., Culvenor, R. A., and Simpson, R. J.: Plant and microbial strategies to improve the phosphorus efficiency of agriculture, Plant Soil, 349, 121–156, 2011.
Roberts, K., Defforey, D., Turner, B. L., Condron, L. M., Peek, S., Silva, S., Kendall, C., and Paytan, A.: Oxygen isotopes of phosphate and soil phosphorus cycling across a 6500 year chronosequence under lowland temperate rainforest, Geoderma, https://doi.org/10.1016/j.geoderma.2015.04.010, 2015.
Shears, S. B. and Turner, B. L.: Nomenclature and terminology of inositol phosphates: clarification and a glossary of terms, in: Inositol Phosphates: Linking Agriculture and the Environment, CAB International, Wallingford, UK, 1–6, 2007.
Stec, B., Holtz, K. M., and Kantrowitz, E. R.: A revised mechanism for the alkaline phosphatase reaction involving three metal ions, J. Mol. Biol., 299, 1303–1311, 2000.
Stout, L. M., Joshi, S. R., Kana, T. M., and Jaisi, D. P.: Microbial activities and phosphorus cycling: An application of oxygen isotope ratios in phosphate, Geochim. Cosmochim. Acta, 138, 101–116, 2014.
Tamburini, F., Bernasconi, S. M., Angert, A., Weiner, T., and Frossard, E.: A method for the analysis of the δ18O of inorganic phosphate extracted from soils with HCl, Eur. J. Soil Sci., 61, 1025–1032, 2010.
Tamburini, F., Pfahler, V., Bünemann, E. K., Guelland, K., Bernasconi, S. M., and Frossard, E.: Oxygen isotopes unravel the role of microorganisms in phosphate cycling in soils, Environ. Sci. Technol., 46, 5956–5962, 2012.
Turner, B. L.: Inositol phosphates in soil: amounts, forms and significance of the phosphorlyated inositol stereoisomers, in: Inositol Phosphates: Linking Agriculture and the Environment, CAB International, Wallingford, UK, 186–207, 2007.
Turner, B. L. and Haygarth, P. M.: Phosphorus forms and concentrations in leachate under four grassland soil types, Soil Sci. Soc. Am. J., 64, 1090–1099, 2000.
Turner, B. L., Papházy, M. J., Haygarth, P. M., and Mckelvie, I. D.: Inositol phosphates in the environment, Phil. Trans. R. Soc. Lond., 357, 449–469, 2002.
van Etten, R. L. V., Davidson, R., Stevis, P. E., MacArthur, H., and Moore, D. L.: Covalent structure, disulfide bonding, and identification of reactive surface and active site residues of human prostatic acid phosphatase, J. Biol. Chem., 266, 2313–2319, 1991.
von Sperber, C., Kries, H., Tamburini, F., Bernasconi, S. M., and Frossard, E.: The effect of phosphomonoesterases on the oxygen isotope composition of phosphate, Geochim. Cosmochim. Acta, 125, 519–527, 2014.
Wodzinski, R. J. and Ullah, A. H. J.: Phytase, in: Advances in Applied Microbiology, edited by: Neidleman, S. L. and Laskin, A. I., Academic Press, 263–302, 1996.
Young, M. B., McLaughlin, K., Kendall, C., Stringfellow, W., Rollog, M., Elsbury, K., Donald, E., and Paytan, A.: Characterizing the oxygen isotopic composition of phosphate sources to aquatic ecosystems, Environ. Sci. Technol., 43, 5190–5196, 2009.
Zimmermann, P., Zardi, G., Lehmann, M., Zeder, C., Amrhein, N., Frossard, E., and Bucher, M.: Engineering the root–soil interface via targeted expression of a synthetic phytase gene in trichoblasts, Plant Biotech. J., 1, 353–360, 2003.
Zohar, I., Shaviv, A., Klass, T., Roberts, K., and Paytan, A.: Method for the analysis of oxygen isotopic composition of soil phosphate fractions, Environ. Sci. Technol., 44, 7583–7588, 2010a.
Zohar, I., Shaviv, A., Young, M., Kendall, C., Silva, S., and Paytan, A.: Phosphorus dynamics in soils irrigated with reclaimed waste water or fresh water – A study using oxygen isotopic composition of phosphate, Geoderma, 159, 109–121, 2010b.