Articles | Volume 11, issue 10
https://doi.org/10.5194/bg-11-2679-2014
© Author(s) 2014. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
https://doi.org/10.5194/bg-11-2679-2014
© Author(s) 2014. This work is distributed under
the Creative Commons Attribution 3.0 License.
the Creative Commons Attribution 3.0 License.
Research article
| 
21 May 2014
Research article |
| 21 May 2014
Isotopomeric characterization of nitrous oxide produced by reaction of enzymes extracted from nitrifying and denitrifying bacteria
T. Yamazaki
Department of Environmental Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan
present address: LSI Medience Corporation, 1000 Kamoshita-chou, Aoba-ku, Yokohama 227-0033, Japan
T. Hozuki
Department of Biological Science, Graduate School of Science, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka 422-8529, Japan
K. Arai
Department of Biological Science, Graduate School of Science, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka 422-8529, Japan
S. Toyoda
Department of Environmental Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan
K. Koba
Institute of Agriculture, Tokyo University of Agriculture and Technology, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-city, Tokyo 183-8509, Japan
T. Fujiwara
Department of Biological Science, Graduate School of Science, Shizuoka University, 836 Oh-ya, Suruga-ku, Shizuoka 422-8529, Japan
N. Yoshida
Earth-Life Science Institute, Tokyo Institute of Technology, Meguro, Tokyo, 152-8551, Japan
Department of Environment Chemistry and Engineering, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan
Department of Environmental Science and Technology, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan
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Kun Wang, Shohei Hattori, Mang Lin, Sakiko Ishino, Becky Alexander, Kazuki Kamezaki, Naohiro Yoshida, and Shichang Kang
Atmos. Chem. Phys., 21, 8357–8376, https://doi.org/10.5194/acp-21-8357-2021, https://doi.org/10.5194/acp-21-8357-2021, 2021
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Sulfate aerosols play an important climatic role and exert adverse effects on the ecological environment and human health. In this study, we present the triple oxygen isotopic composition of sulfate from the Mt. Everest region, southern Tibetan Plateau, and decipher the formation mechanisms of atmospheric sulfate in this pristine environment. The results indicate the important role of the S(IV) + O3 pathway in atmospheric sulfate formation promoted by conditions of high cloud water pH.
Tamaki Fujinawa, Tomohiro O. Sato, Takayoshi Yamada, Seidai Nara, Yuki Uchiyama, Kodai Takahashi, Naohiro Yoshida, and Yasuko Kasai
Atmos. Meas. Tech., 13, 2119–2129, https://doi.org/10.5194/amt-13-2119-2020, https://doi.org/10.5194/amt-13-2119-2020, 2020
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We performed an error analysis of SMILES observations for acetonitrile and a validation using the MLS observations by extracting the coincident points between SMILES and MLS data. The major error sources for the SMILES observations were quantitatively estimated. At upper pressure levels the difference between the two datasets increased because of an uncertainty in MLS observations. The results showed that SMILES has an advantage in measuring acetonitrile in the upper stratosphere and mesosphere.
Kazuki Kamezaki, Shohei Hattori, Enno Bahlmann, and Naohiro Yoshida
Atmos. Meas. Tech., 12, 1141–1154, https://doi.org/10.5194/amt-12-1141-2019, https://doi.org/10.5194/amt-12-1141-2019, 2019
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Knowledge related to sulfur isotopic composition of carbonyl sulfide (OCS or COS), the most abundant atmospheric sulfur species, remains scarce. We present a new sampling system for collecting approx. 10 nmol of OCS from ambient air coupled with a purification system. The system presented herein is useful for application of sulfur isotopic compositions for investigation of OCS sources and sinks in the troposphere to elucidate its cycle and its contribution to background stratospheric sulfate.
Satoshi Sugawara, Shigeyuki Ishidoya, Shuji Aoki, Shinji Morimoto, Takakiyo Nakazawa, Sakae Toyoda, Yoichi Inai, Fumio Hasebe, Chusaku Ikeda, Hideyuki Honda, Daisuke Goto, and Fanny A. Putri
Atmos. Chem. Phys., 18, 1819–1833, https://doi.org/10.5194/acp-18-1819-2018, https://doi.org/10.5194/acp-18-1819-2018, 2018
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This is the first research that shows concrete evidence of gravitational separation in the tropical stratosphere. This implies that gravitational separation occurs within the entire stratosphere, which gives us new insight into atmospheric dynamics.
Sakae Toyoda, Naohiro Yoshida, Shinji Morimoto, Shuji Aoki, Takakiyo Nakazawa, Satoshi Sugawara, Shigeyuki Ishidoya, Mitsuo Uematsu, Yoichi Inai, Fumio Hasebe, Chusaku Ikeda, Hideyuki Honda, and Kentaro Ishijima
Atmos. Chem. Phys., 18, 833–844, https://doi.org/10.5194/acp-18-833-2018, https://doi.org/10.5194/acp-18-833-2018, 2018
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By analysis of whole air samples collected by balloon-borne compact cryogenic samplers, we found that apparent isotope effect for stratospheric N2O between 25 and 30 km over the Equator is larger than that observed in other latitudes and that it is almost equal to the effect predicted by laboratory simulation experiments. These results suggest that equatorial middle stratosphere can be treated as an isolated region when we consider the decomposition of N2O by photochemical processes.
Sakiko Ishino, Shohei Hattori, Joel Savarino, Bruno Jourdain, Susanne Preunkert, Michel Legrand, Nicolas Caillon, Albane Barbero, Kota Kuribayashi, and Naohiro Yoshida
Atmos. Chem. Phys., 17, 3713–3727, https://doi.org/10.5194/acp-17-3713-2017, https://doi.org/10.5194/acp-17-3713-2017, 2017
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We show the first simultaneous observations of triple oxygen isotopic compositions of atmospheric sulfate, nitrate, and ozone at Dumont d'Urville, coastal Antarctica. The contrasting seasonal trends between oxygen isotopes of ozone and those of sulfate and nitrate indicate that these signatures in sulfate and nitrate are mainly controlled by changes in oxidation chemistry. We also discuss the specific oxidation chemistry induced by the unique phenomena at the site.
Sakae Toyoda and Naohiro Yoshida
Atmos. Meas. Tech., 9, 2093–2101, https://doi.org/10.5194/amt-9-2093-2016, https://doi.org/10.5194/amt-9-2093-2016, 2016
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Tiny variation in natural abundance ratios of stable isotopes of nitrogen and oxygen in nitrous oxide (N2O) can be used as a fingerprint of this trace gas that causes greenhouse effect and stratospheric ozone depletion. Toward the understanding of the global budget of N2O and its temporal change, we developed an automated sample preparation system that can be used to measure the stable isotope ratios in N2O in various air samples collected into flasks with high precision and less labor.
Koki Maeda, Sakae Toyoda, Midori Yano, Shohei Hattori, Makoto Fukasawa, Keiichi Nakajima, and Naohiro Yoshida
Biogeosciences, 13, 1341–1349, https://doi.org/10.5194/bg-13-1341-2016, https://doi.org/10.5194/bg-13-1341-2016, 2016
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Nitrogen isotope ratios (δ15N) of NH4+ in dairy manure compost piles were studied. The δ15N–NH4+ values in different pile zones (top, side and core) show that extremely high nitrogen conversion, nitrification–denitrification activity of the microbes and NH3 volatilization occurred in pile top zone, which has very high NH4+ concentrations and significantly high 15N (δ15N: 12.7–29.8 ‰) values.
K. Ishijima, M. Takigawa, K. Sudo, S. Toyoda, N. Yoshida, T. Röckmann, J. Kaiser, S. Aoki, S. Morimoto, S. Sugawara, and T. Nakazawa
Atmos. Chem. Phys. Discuss., https://doi.org/10.5194/acpd-15-19947-2015, https://doi.org/10.5194/acpd-15-19947-2015, 2015
Revised manuscript not accepted
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We developed an atmospheric N2O isotopocule model based on a chemistry-coupled atmospheric general circulation model and a simple method to optimize the model, and estimated the isotopic signatures of surface sources at the hemispheric scale. Data obtained from ground-based observations, measurements of firn air, and balloon and aircraft flights were used to optimize the long-term trends, interhemispheric gradients, and photolytic fractionation, respectively, in the model.
N. Zhang, K. Yamada, N. Suzuki, and N. Yoshida
Biogeosciences, 11, 5335–5348, https://doi.org/10.5194/bg-11-5335-2014, https://doi.org/10.5194/bg-11-5335-2014, 2014
T. O. Sato, H. Sagawa, N. Yoshida, and Y. Kasai
Atmos. Meas. Tech., 7, 941–958, https://doi.org/10.5194/amt-7-941-2014, https://doi.org/10.5194/amt-7-941-2014, 2014
J. A. Schmidt, M. S. Johnson, S. Hattori, N. Yoshida, S. Nanbu, and R. Schinke
Atmos. Chem. Phys., 13, 1511–1520, https://doi.org/10.5194/acp-13-1511-2013, https://doi.org/10.5194/acp-13-1511-2013, 2013
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Robin Havas, Christophe Thomazo, Miguel Iniesto, Didier Jézéquel, David Moreira, Rosaluz Tavera, Jeanne Caumartin, Elodie Muller, Purificación López-García, and Karim Benzerara
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Biogeosciences, 20, 205–227, https://doi.org/10.5194/bg-20-205-2023, https://doi.org/10.5194/bg-20-205-2023, 2023
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Marta Santos-Garcia, Raja S. Ganeshram, Robyn E. Tuerena, Margot C. F. Debyser, Katrine Husum, Philipp Assmy, and Haakon Hop
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Marlene Dordoni, Michael Seewald, Karsten Rinke, Kurt Friese, Robert van Geldern, Jakob Schmidmeier, and Johannes A. C. Barth
Biogeosciences, 19, 5343–5355, https://doi.org/10.5194/bg-19-5343-2022, https://doi.org/10.5194/bg-19-5343-2022, 2022
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Frédérique M. S. A. Kirkels, Hugo J. de Boer, Paulina Concha Hernández, Chris R. T. Martes, Marcel T. J. van der Meer, Sayak Basu, Muhammed O. Usman, and Francien Peterse
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The distinct carbon isotopic values of C3 and C4 plants are widely used to reconstruct past hydroclimate, where more C3 plants reflect wetter and C4 plants drier conditions. Here we examine the impact of regional hydroclimatic conditions on plant isotopic values in the Godavari River basin, India. We find that it is crucial to identify regional plant isotopic values and consider drought stress, which introduces a bias in C3 / C4 plant estimates and associated hydroclimate reconstructions.
Mhlangabezi Mdutyana, Tanya Marshall, Xin Sun, Jessica M. Burger, Sandy J. Thomalla, Bess B. Ward, and Sarah E. Fawcett
Biogeosciences, 19, 3425–3444, https://doi.org/10.5194/bg-19-3425-2022, https://doi.org/10.5194/bg-19-3425-2022, 2022
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Nitrite-oxidizing bacteria in the winter Southern Ocean show a high affinity for nitrite but require a minimum (i.e., "threshold") concentration before they increase their rates of nitrite oxidation significantly. The classic Michaelis–Menten model thus cannot be used to derive the kinetic parameters, so a modified equation was employed that also yields the threshold nitrite concentration. Dissolved iron availability may play an important role in limiting nitrite oxidation.
Weitian Ding, Urumu Tsunogai, Fumiko Nakagawa, Takashi Sambuichi, Hiroyuki Sase, Masayuki Morohashi, and Hiroki Yotsuyanagi
Biogeosciences, 19, 3247–3261, https://doi.org/10.5194/bg-19-3247-2022, https://doi.org/10.5194/bg-19-3247-2022, 2022
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Excessive leaching of nitrate from forested catchments during storm events degrades water quality and causes eutrophication in downstream areas. Thus, tracing the source of nitrate increase during storm events in forested streams is important for sustainable forest management. Based on the isotopic compositions of stream nitrate, including Δ17O, this study clarifies that the source of stream nitrate increase during storm events was soil nitrate in the riparian zone.
Nicolas Séon, Romain Amiot, Guillaume Suan, Christophe Lécuyer, François Fourel, Fabien Demaret, Arnauld Vinçon-Laugier, Sylvain Charbonnier, and Peggy Vincent
Biogeosciences, 19, 2671–2681, https://doi.org/10.5194/bg-19-2671-2022, https://doi.org/10.5194/bg-19-2671-2022, 2022
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We analysed the oxygen isotope composition of bones and teeth of four marine species possessing regional heterothermies. We observed a consistent link between oxygen isotope composition and temperature heterogeneities recorded by classical methods. This opens up new perspectives on the determination of the thermoregulatory strategies of extant marine vertebrates where conventional methods are difficult to apply, but also allows us to investigate thermophysiologies of extinct vertebrates.
Yuwei Liu, Guofeng Zhu, Zhuanxia Zhang, Zhigang Sun, Leilei Yong, Liyuan Sang, Lei Wang, and Kailiang Zhao
Biogeosciences, 19, 877–889, https://doi.org/10.5194/bg-19-877-2022, https://doi.org/10.5194/bg-19-877-2022, 2022
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We took the water cycle process of soil–plant–atmospheric precipitation as the research objective. In the water cycle of soil–plant–atmospheric precipitation, precipitation plays the main controlling role. The main source of replenishment for alpine meadow plants is precipitation and alpine meltwater; the main source of replenishment for forest plants is soil water; and the plants in the arid foothills mainly use groundwater.
Roberto Velázquez-Ochoa, María Julia Ochoa-Izaguirre, and Martín Federico Soto-Jiménez
Biogeosciences, 19, 1–27, https://doi.org/10.5194/bg-19-1-2022, https://doi.org/10.5194/bg-19-1-2022, 2022
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Our research is the first approximation to understand the δ13C macroalgal variability in one of the most diverse marine ecosystems in the world, the Gulf of California. The life-form is the principal cause of δ13C macroalgal variability, mainly taxonomy. However, changes in habitat characteristics and environmental conditions also influence the δ13C macroalgal variability. The δ13C macroalgae is indicative of carbon concentration mechanisms and isotope discrimination during carbon assimilation.
Raquel F. Flynn, Thomas G. Bornman, Jessica M. Burger, Shantelle Smith, Kurt A. M. Spence, and Sarah E. Fawcett
Biogeosciences, 18, 6031–6059, https://doi.org/10.5194/bg-18-6031-2021, https://doi.org/10.5194/bg-18-6031-2021, 2021
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Biological activity in the shallow Weddell Sea affects the biogeochemistry of recently formed deep waters. To investigate the drivers of carbon and nutrient export, we measured rates of primary production and nitrogen uptake, characterized the phytoplankton community, and estimated nutrient depletion ratios across the under-sampled western Weddell Sea in mid-summer. Carbon export was highest at the ice shelves and was determined by a combination of physical, chemical, and biological factors.
Stéphanie H. M. Jacquet, Christian Tamburini, Marc Garel, Aurélie Dufour, France Van Vambeke, Frédéric A. C. Le Moigne, Nagib Bhairy, and Sophie Guasco
Biogeosciences, 18, 5891–5902, https://doi.org/10.5194/bg-18-5891-2021, https://doi.org/10.5194/bg-18-5891-2021, 2021
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We compared carbon remineralization rates (MRs) in the western and central Mediterranean Sea in late spring during the PEACETIME cruise, as assessed using the barium tracer. We reported higher and deeper (up to 1000 m depth) MRs in the western basin, potentially sustained by an additional particle export event driven by deep convection. The central basin is the site of a mosaic of blooming and non-blooming water masses and showed lower MRs that were restricted to the upper mesopelagic layer.
Shinsuke Kawagucci, Yohei Matsui, Akiko Makabe, Tatsuhiro Fukuba, Yuji Onishi, Takuro Nunoura, and Taichi Yokokawa
Biogeosciences, 18, 5351–5362, https://doi.org/10.5194/bg-18-5351-2021, https://doi.org/10.5194/bg-18-5351-2021, 2021
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Hydrogen and carbon isotope ratios of methane as well as the relevant biogeochemical parameters and microbial community compositions in hydrothermal plumes in the Okinawa Trough were observed. We succeeded in simultaneously determining hydrogen and carbon isotope fractionation factors associated with aerobic oxidation of methane in seawater (εH = 49.4 ± 5.0 ‰, εC = 5.2 ± 0.4 ‰) – the former being the first of its kind ever reported.
Nicolai Schleinkofer, David Evans, Max Wisshak, Janina Vanessa Büscher, Jens Fiebig, André Freiwald, Sven Härter, Horst R. Marschall, Silke Voigt, and Jacek Raddatz
Biogeosciences, 18, 4733–4753, https://doi.org/10.5194/bg-18-4733-2021, https://doi.org/10.5194/bg-18-4733-2021, 2021
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We have measured the chemical composition of the carbonate shells of the parasitic foraminifera Hyrrokkin sarcophaga in order to test if it is influenced by the host organism (bivalve or coral). We find that both the chemical and isotopic composition is influenced by the host organism. For example strontium is enriched in foraminifera that grew on corals, whose skeleton is built from aragonite, which is naturally enriched in strontium compared to the bivalves' calcite shell.
Lena Rohe, Traute-Heidi Anderson, Heinz Flessa, Anette Goeske, Dominika Lewicka-Szczebak, Nicole Wrage-Mönnig, and Reinhard Well
Biogeosciences, 18, 4629–4650, https://doi.org/10.5194/bg-18-4629-2021, https://doi.org/10.5194/bg-18-4629-2021, 2021
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This is the first experimental setup combining a complex set of methods (microbial inhibitors and isotopic approaches) to differentiate between N2O produced by fungi or bacteria during denitrification in three soils. Quantifying the fungal fraction with inhibitors was not successful due to large amounts of uninhibited N2O production. All successful methods suggested a small or missing fungal contribution. Artefacts occurring with microbial inhibition to determine N2O fluxes are discussed.
Inga Köhler, Raul E. Martinez, David Piatka, Achim J. Herrmann, Arianna Gallo, Michelle M. Gehringer, and Johannes A. C. Barth
Biogeosciences, 18, 4535–4548, https://doi.org/10.5194/bg-18-4535-2021, https://doi.org/10.5194/bg-18-4535-2021, 2021
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We investigated how high Fe(II) levels influence the O2 budget of a circum-neutral Fe(II)-rich spring and if a combined study of dissolved O (DO) and its isotopic composition can help assess this effect. We showed that dissolved Fe(II) can exert strong effects on the δ18ODO even though a constant supply of atmospheric O2 occurs. In the presence of photosynthesis, direct effects of Fe oxidation become masked. Critical Fe(II) concentrations indirectly control the DO by enhancing photosynthesis.
Owen A. Sherwood, Samuel H. Davin, Nadine Lehmann, Carolyn Buchwald, Evan N. Edinger, Moritz F. Lehmann, and Markus Kienast
Biogeosciences, 18, 4491–4510, https://doi.org/10.5194/bg-18-4491-2021, https://doi.org/10.5194/bg-18-4491-2021, 2021
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Pacific water flowing eastward through the Canadian Arctic plays an important role in redistributing nutrients to the northwest Atlantic Ocean. Using samples collected from northern Baffin Bay to the southern Labrador Shelf, we show that stable isotopic ratios in seawater nitrate reflect the fraction of Pacific to Atlantic water. These results provide a new framework for interpreting patterns of nitrogen isotopic variability recorded in modern and archival organic materials in the region.
Franziska Slotta, Lukas Wacker, Frank Riedel, Karl-Uwe Heußner, Kai Hartmann, and Gerhard Helle
Biogeosciences, 18, 3539–3564, https://doi.org/10.5194/bg-18-3539-2021, https://doi.org/10.5194/bg-18-3539-2021, 2021
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The African baobab is a challenging climate and environmental archive for its semi-arid habitat due to dating uncertainties and parenchyma-rich wood anatomy. Annually resolved F14C data of tree-ring cellulose (1941–2005) from a tree in Oman show the annual character of the baobab’s growth rings but were up to 8.8 % lower than expected for 1964–1967. Subseasonal δ13C and δ18O patterns reveal years with low average monsoon rain as well as heavy rainfall events from pre-monsoonal cyclones.
Peter M. J. Douglas, Emerald Stratigopoulos, Sanga Park, and Dawson Phan
Biogeosciences, 18, 3505–3527, https://doi.org/10.5194/bg-18-3505-2021, https://doi.org/10.5194/bg-18-3505-2021, 2021
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Hydrogen isotopes could be a useful tool to help resolve the geographic distribution of methane emissions from freshwater environments. We analyzed an expanded global dataset of freshwater methane hydrogen isotope ratios and found significant geographic variation linked to water isotopic composition. This geographic variability could be used to resolve changing methane fluxes from freshwater environments and provide more accurate estimates of the relative balance of global methane sources.
Veronica R. Rollinson, Julie Granger, Sydney C. Clark, Mackenzie L. Blanusa, Claudia P. Koerting, Jamie M. P. Vaudrey, Lija A. Treibergs, Holly C. Westbrook, Catherine M. Matassa, Meredith G. Hastings, and Craig R. Tobias
Biogeosciences, 18, 3421–3444, https://doi.org/10.5194/bg-18-3421-2021, https://doi.org/10.5194/bg-18-3421-2021, 2021
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We measured nutrients and the naturally occurring nitrogen (N) and oxygen (O) stable isotope ratios of nitrate discharged from a New England river over an annual cycle, to monitor N loading and identify dominant sources from the watershed. We uncovered a seasonality to loading and sources of N from the watershed. Seasonality in the nitrate isotope ratios also informed on N cycling, conforming to theoretical expectations of riverine nutrient cycling.
Zixun Chen, Xuejun Liu, Xiaoqing Cui, Yaowen Han, Guoan Wang, and Jiazhu Li
Biogeosciences, 18, 2859–2870, https://doi.org/10.5194/bg-18-2859-2021, https://doi.org/10.5194/bg-18-2859-2021, 2021
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δ13C in plants is a sensitive long-term indicator of physiological acclimatization. The present study suggests that precipitation change and increasing atmospheric N deposition have little impact on δ13C of H. ammodendron, a dominant plant in central Asian deserts, but affect its gas exchange. In addition, this study shows that δ13C of H. ammodendron could not indicate its water use efficiency (WUE), suggesting that whether δ13C of C4 plants indicates WUE is species-specific.
Petra Zahajská, Carolina Olid, Johanna Stadmark, Sherilyn C. Fritz, Sophie Opfergelt, and Daniel J. Conley
Biogeosciences, 18, 2325–2345, https://doi.org/10.5194/bg-18-2325-2021, https://doi.org/10.5194/bg-18-2325-2021, 2021
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The drivers of high accumulation of single-cell siliceous algae (diatoms) in a high-latitude lake have not been fully characterized before. We studied silicon cycling of the lake through water, radon, silicon, and stable silicon isotope balances. Results showed that groundwater brings 3 times more water and dissolved silica than the stream inlet. We demonstrate that groundwater discharge and low sediment deposition have driven the high diatom accumulation in the studied lake in the past century.
Yu-Te Hsieh, Walter Geibert, E. Malcolm S. Woodward, Neil J. Wyatt, Maeve C. Lohan, Eric P. Achterberg, and Gideon M. Henderson
Biogeosciences, 18, 1645–1671, https://doi.org/10.5194/bg-18-1645-2021, https://doi.org/10.5194/bg-18-1645-2021, 2021
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The South Atlantic near 40° S is one of the high-productivity and most dynamic nutrient regions in the oceans, but the sources and fluxes of trace elements (TEs) to this region remain unclear. This study investigates seawater Ra-228 and provides important constraints on ocean mixing and dissolved TE fluxes to this region. Vertical mixing is a more important source than aeolian or shelf inputs in this region, but particulate or winter deep-mixing inputs may be required to balance the TE budgets.
Zhongjie Yu and Emily M. Elliott
Biogeosciences, 18, 805–829, https://doi.org/10.5194/bg-18-805-2021, https://doi.org/10.5194/bg-18-805-2021, 2021
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In this study, we demonstrated distinct nitrogen isotope effects for nitric oxide (NO) production from major microbial and chemical NO sources in an agricultural soil. These results highlight characteristic bond-forming and breaking mechanisms associated with microbial and chemical NO production and implicate that simultaneous isotopic analyses of NO and nitrous oxide (N2O) can lead to unprecedented insights into the sources and processes controlling NO and N2O emissions from agricultural soils.
Daniel A. Frick, Rainer Remus, Michael Sommer, Jürgen Augustin, Danuta Kaczorek, and Friedhelm von Blanckenburg
Biogeosciences, 17, 6475–6490, https://doi.org/10.5194/bg-17-6475-2020, https://doi.org/10.5194/bg-17-6475-2020, 2020
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Silicon is taken up by some plants to increase structural stability and to develop stress resistance and is rejected by others. To explore the underlying mechanisms, we used the stable isotopes of silicon that shift in their relative abundance depending on the biochemical transformation involved. On species with a rejective (tomato, mustard) and active (wheat) uptake mechanism, grown in hydroculture, we found that the transport of silicic acid is controlled by the precipitation of biogenic opal.
Quentin Charbonnier, Julien Bouchez, Jérôme Gaillardet, and Éric Gayer
Biogeosciences, 17, 5989–6015, https://doi.org/10.5194/bg-17-5989-2020, https://doi.org/10.5194/bg-17-5989-2020, 2020
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The abundance and isotope composition of the trace metal barium (Ba) allows us to track and quantify nutrient cycling throughout the Amazon Basin. In particular, we show that the Ba biological fingerprint evolves from that of a strong net nutrient uptake in the mountainous area of the Andes towards efficient nutrient recycling on the plains of the Lower Amazon. Our study highlights the fact that the geochemical signature of rock-derived nutrients transported by the Amazon is scarred by life.
Ajinkya G. Deshpande, Thomas W. Boutton, Ayumi Hyodo, Charles W. Lafon, and Georgianne W. Moore
Biogeosciences, 17, 5639–5653, https://doi.org/10.5194/bg-17-5639-2020, https://doi.org/10.5194/bg-17-5639-2020, 2020
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Wetland forests in the southern USA are threatened by changing climate and human-induced pressures. We used tree ring widths and C isotopes as indicators of forest growth and physiological stress, respectively, and compared these to past climate data. We observed that vegetation growing in the drier patches is susceptible to stress, while vegetation growth and physiology in wetter patches is less sensitive to unfavorable environmental conditions, highlighting the importance of optimal wetness.
Dominika Lewicka-Szczebak, Maciej Piotr Lewicki, and Reinhard Well
Biogeosciences, 17, 5513–5537, https://doi.org/10.5194/bg-17-5513-2020, https://doi.org/10.5194/bg-17-5513-2020, 2020
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We present the first validation of N2O isotopic approaches for estimating N2O source pathways and N2O reduction. These approaches are widely used for tracing soil nitrogen cycling, but the results of these estimations are very uncertain. Here we report the results from parallel treatments allowing for precise validation of these approaches, and we propose the best strategies for results interpretation, including the new idea of an isotope model integrating three isotopic signatures of N2O.
Markus Raitzsch, Claire Rollion-Bard, Ingo Horn, Grit Steinhoefel, Albert Benthien, Klaus-Uwe Richter, Matthieu Buisson, Pascale Louvat, and Jelle Bijma
Biogeosciences, 17, 5365–5375, https://doi.org/10.5194/bg-17-5365-2020, https://doi.org/10.5194/bg-17-5365-2020, 2020
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The isotopic composition of boron in carbonate shells of marine unicellular organisms is a popular tool to estimate seawater pH. Usually, many shells need to be dissolved and measured for boron isotopes, but the information on their spatial distribution is lost. Here, we investigate two techniques that allow for measuring boron isotopes within single shells and show that they yield robust mean values but provide additional information on the heterogeneity within and between single shells.
Florian Einsiedl, Anja Wunderlich, Mathieu Sebilo, Ömer K. Coskun, William D. Orsi, and Bernhard Mayer
Biogeosciences, 17, 5149–5161, https://doi.org/10.5194/bg-17-5149-2020, https://doi.org/10.5194/bg-17-5149-2020, 2020
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Nitrate pollution of freshwaters and methane emissions into the atmosphere are crucial factors in deteriorating the quality of drinking water and in contributing to global climate change. Here, we report vertical concentration and stable isotope profiles of CH4, NO3-, NO2-, and NH4+ in the water column of Fohnsee (southern Bavaria, Germany) that may indicate linkages between nitrate-dependent anaerobic methane oxidation and the anaerobic oxidation of ammonium.
Ruifang C. Xie, Frédéric A. C. Le Moigne, Insa Rapp, Jan Lüdke, Beat Gasser, Marcus Dengler, Volker Liebetrau, and Eric P. Achterberg
Biogeosciences, 17, 4919–4936, https://doi.org/10.5194/bg-17-4919-2020, https://doi.org/10.5194/bg-17-4919-2020, 2020
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Thorium-234 (234Th) is widely used to study carbon fluxes from the surface ocean to depth. But few studies stress the relevance of oceanic advection and diffusion on the downward 234Th fluxes in nearshore environments. Our study in offshore Peru showed strong temporal variations in both the importance of physical processes on 234Th flux estimates and the oceanic residence time of 234Th, whereas salinity-derived seawater 238U activities accounted for up to 40 % errors in 234Th flux estimates.
Ralf A. Oeser and Friedhelm von Blanckenburg
Biogeosciences, 17, 4883–4917, https://doi.org/10.5194/bg-17-4883-2020, https://doi.org/10.5194/bg-17-4883-2020, 2020
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We present a novel strategy to decipher the relative impact of biogenic and abiotic drivers of weathering. We parameterized the nutrient fluxes in four ecosystems along a climate and vegetation gradient situated on the Chilean Coastal Cordillera. We investigated how nutrient demand by plants drives weathering. We found that the increase in biomass nutrient demand is accommodated by faster nutrient recycling rather than an increase in the weathering–release rates.
Tito Arosio, Malin M. Ziehmer, Kurt Nicolussi, Christian Schlüchter, and Markus Leuenberger
Biogeosciences, 17, 4871–4882, https://doi.org/10.5194/bg-17-4871-2020, https://doi.org/10.5194/bg-17-4871-2020, 2020
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Stable isotopes in tree-ring cellulose are tools for climatic reconstructions, but interpretation is challenging due to nonclimate trends. We analyzed the tree-age trends in tree-ring isotopes of deciduous larch and evergreen cembran pine. Samples covering the whole Holocene were collected at the tree line in the Alps. For cambial ages over 100 years, we prove the absence of age trends in δD, δ18O, and δ13C for both species. For lower cambial ages, trends differ for each isotope and species.
Yuyang He, Xiaobin Cao, and Huiming Bao
Biogeosciences, 17, 4785–4795, https://doi.org/10.5194/bg-17-4785-2020, https://doi.org/10.5194/bg-17-4785-2020, 2020
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Different carbon sites in a large organic molecule have different isotope compositions. Different carbon sites may not have the chance to exchange isotopes at all. The lack of appreciation of this notion might be blamed for an unsettled debate on the thermodynamic state of an organism. Here we demonstrate using minerals, N2O, and acetic acid that the dearth of exchange among different carbon sites renders them as independent as if they were different elements in organic molecules.
Felix M. Spielmann, Albin Hammerle, Florian Kitz, Katharina Gerdel, and Georg Wohlfahrt
Biogeosciences, 17, 4281–4295, https://doi.org/10.5194/bg-17-4281-2020, https://doi.org/10.5194/bg-17-4281-2020, 2020
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Carbonyl sulfide (COS) can be used as a proxy for plant photosynthesis on an ecosystem scale. However, the relationships between COS and CO2 fluxes and their dependence on daily to seasonal changes in environmental drivers are still poorly understood. We examined COS and CO2 ecosystem fluxes above an agriculturally used mountain grassland for 6 months. Harvesting of the grassland disturbed the otherwise stable COS-to-CO2 uptake ratio. We even found the canopy to release COS during those times.
Getachew Agmuas Adnew, Thijs L. Pons, Gerbrand Koren, Wouter Peters, and Thomas Röckmann
Biogeosciences, 17, 3903–3922, https://doi.org/10.5194/bg-17-3903-2020, https://doi.org/10.5194/bg-17-3903-2020, 2020
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We measured the effect of photosynthesis, the largest flux in the carbon cycle, on the triple oxygen isotope composition of atmospheric CO2 at the leaf level during gas exchange using three plant species. The main factors that limit the impact of land vegetation on the triple oxygen isotope composition of atmospheric CO2 are identified, characterized and discussed. The effect of photosynthesis on the isotopic composition of CO2 is commonly quantified as discrimination (ΔA).
Moritz Schroll, Frank Keppler, Markus Greule, Christian Eckhardt, Holger Zorn, and Katharina Lenhart
Biogeosciences, 17, 3891–3901, https://doi.org/10.5194/bg-17-3891-2020, https://doi.org/10.5194/bg-17-3891-2020, 2020
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Fungi have recently been identified to produce the greenhouse gas methane. Here, we investigated the stable carbon isotope values of methane produced by saprotrophic fungi. Our results show that stable isotope values of methane from fungi are dependent on the fungal species and the metabolized substrate. They cover a broad range and overlap with stable carbon isotope values of methane reported for methanogenic archaea, the thermogenic degradation of organic matter, and other eukaryotes.
Pranav Hirave, Guido L. B. Wiesenberg, Axel Birkholz, and Christine Alewell
Biogeosciences, 17, 2169–2180, https://doi.org/10.5194/bg-17-2169-2020, https://doi.org/10.5194/bg-17-2169-2020, 2020
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Sediment input into water bodies is a prominent threat to freshwater ecosystems. We tested the stability of tracers employed in freshwater sediment tracing based on compound-specific isotope analysis during early degradation in soil. While bulk δ13C values showed no stability, δ13C values of plant-derived fatty acids and n-alkanes were stably transferred to the soil without soil particle size dependency after an early degradation in organic horizons, thus indicating their suitability as tracers.
Caroline Thaler, Amandine Katz, Magali Bonifacie, Bénédicte Ménez, and Magali Ader
Biogeosciences, 17, 1731–1744, https://doi.org/10.5194/bg-17-1731-2020, https://doi.org/10.5194/bg-17-1731-2020, 2020
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Paleoenvironment reconstructions, retrieved from δ18O and Δ47 values measured in carbonate, are compromised when crystallization occurs in isotopic disequilibrium. We show that some paleoenvironmental information can still be retrieved from these paired disequilibrium Δ47 and δ18O values. The possibility of retrieving information on paleowaters, sediments' interstitial waters, or organisms' body water at the carbonate precipitation loci will help understand past Earth and life evolution.
Cited articles
Arp, D. J. and Stein, L. Y.: Metabolism of inorganic N compounds by ammonia-oxidizing bacteria, Crit. Rev. Biochem. Mol. Biol., 38, 471–495, 2003.
Baggs, E. M.: A review of stable isotope techniques for N2O source partitioning in soils: recent progress, remaining challenges and future considerations, Rapid Commun. Mass. Sp., 22, 1664–1672, 2008.
Beaumont, H. J., Lens, S. I., Reijnders, W., Westerhoff, H. V., and van Spanning, R. J.: Expression of nitrite reductase in Nitrosomonas europaea involves NsrR, a novel nitrite-sensitive transcription repressor, Mol. Microbiol., 54, 148–158, https://doi.org/10.1111/j.1365-2958.2004.04248.x, 2004.
Casciotti, K., Buchwald, C., Santoro, A. E., and Frame, C.: Assessment of nitrogen and oxygen isotopic fractionation during nitrification and itsexpression in the marine environment, Methods Enzymol., 486, 253–280, https://doi.org/10.1016/B978-0-12-381294-0.00011-0, 2011.
Frame, C. H. and Casciotti, K. L.: Biogeochemical controls and isotopic signatures of nitrous oxide production by a marine ammonia-oxidizing bacterium, Biogeosciences, 7, 2695–2709, https://doi.org/10.5194/bgd-7-3019-2010, 2010.
Fujiwara, T. and Fukumori, Y.: Cytochrome cb-type nitric oxide reductase with cytochrome c oxidase activity from Paracoccus denitrificans ATCC 35512, J. Bacteriol., 178, 1866–1871, 1996.
Goldberg, S. D., Borken, W., and Gebauer, G.: N2O emission in a Norway spruce forest due to soil frost: concentration and isotope profiles shed a new light on an old story, Biochem., 97, 21–30, https://doi.org/10.1007/s10533-009-9294-z, 2010.
Gundersen, K.: The growth and respiration of Nitrosocystis oceanus at different partial pressures of oxygen, J. Gen. Microbiol., 42, 387–396, 1966.
Hayatu, M., Tago, K., and Saito, M.: Various players in the nitrogen cycle; Diversity and functions of the microorganisms involved in nitrification and denitrification, Soil Sci. Plant Nutri., 54, 33-45, https://doi.org/10.1111/j.1747-0765.2007.00195.x, 2008.
Hino, T., Matsumoto, Y., Nagano, S., Sugimoto, H., Fukumori, Y., Murata, T., Iwata, S., and Shiro, Y.: Structural Basis of Biological N2O Generation by Bacterial Nitric Oxide Reductase, Science, 330, 1666, https://doi.org/10.1126/science.1195591, 2010.
Hendriks. J., Oubrie, A., Castresana, J., Urbani, A., Gemeinhardt, S., and Saraste, M.: Nitric oxide reductases in bacteria, Biochim. Biophys. Ac., 1459, 266–273, 2000.
Hozuki, T., Ohtsuka, T., Arai, K., Yoshimatsu, K., Tanaka, S., and Fujiwara, T.: Effect of salinity on hydroxylamine-oxidation in marine ammonia-oxidizing γ-proteobacterium, Nitrosococcus oceani: molecular and catalytic properties of tetraheme cytochrome c-554, Microbes Environ., 25, 95–102, https://doi.org/10.1264/jsme2.ME09154, 2010.
Igarashi, N., Moriyama, H., Fujiwara, T., Fukumori, Y., and Tanaka, N.: The 2.8 Å structure of hydroxylamine oxidoreductase from a nitrifying chemoautotrophic bacterium, Nitrosomonas europaea, Nat. Struct. Biol., 4, 276–284, https://doi.org/10.1038/nsb0497-276, 1997.
Koba, K., Osaka, K., Tobari, Y., Toyoda, S., Ohte, N., Katsuyama, M., Suzuki, N., Itoh, M., Yamagishi, H., Kawasaki, M., Kim, S. J., Yoshida, N., and Nakajima, T.: Biogeochemistry of nitrous oxide in groundwater in a forested ecosystem elucidated by nitrous oxide isotopomer measurements, Geochim. Cosmochim. Ac., 73, 3115–3133, https://doi.org/10.1016/j.gca.2009.03.022, 2009.
Klotz, M. G., Arp, D. J., Chain, P. S. G., El-Sheikh, A. F., Hauser, L. J., Hommes, N. G., Larimer, F. W., Malfatti, S. A., Norton, J. M., Poret-Peterson, A. T., Vergez, L. M., and Ward, B. B.: Complete genome sequence of the marine, chemolithoautotrophic, ammonia-oxidizing bacterium Nitrosococcus oceani ATCC 19707, Appl. Environ. Microbiol., 72, 6299–6315, https://doi.org/10.1128/AEM.00463-06, 2006.
Maalcke, W. J., Dietl, A., Marritt, S. J., Butt, J. N., Jetten, M. S. M., Keltjens, J. T., Barends, T. R. M., and Kartal, B.: Structural basis of biological NO generation by octaheme oxidoreductases, J. Biol. Chem., 289, 1228–1242, https://doi.org/10.1074/jbc.M113.525147, 2014.
Nicholas, D. J. D. and Mason, A.: Determination of nitrate and nitrite, Methods Enzymol., 3, 981–984, 1957.
Numata, M., Saito, T., Yamazaki,T., Fukumori, Y., and Yamanaka, T.: Cytochrome P-460 of Nitrosomonas europaea: further purification and further characterization., J. Biochem., 108, 1016–1023, 1990.
Park, S., Pérez, T., Boering, K. A., Trumbore, S. E., Gil, J., Marquina, S., and Tyler, S. C.: Can N2O stable isotopes and isotopomers be useful tools to characterize sources and microbial pathways of N2O production and consumption in tropical soils?, Global Biogeochem. Cy., 25, GB1001, https://doi.org/10.1029/2009GB003615, 2011.
Poth, M. and Focht, D.:15N kinetic analysis of N2O production by Nitrosomonas europaea: an examination of nitrifier denitrification, Appl. Environ. Microbiol., 49, 1134–1141, 1985.
Ravishankara, A. R., Daniel, J., and Sortman, R. W.: Nitrous oxide (N2O): The dominant ozone-depleting substance emitted in the 21st century, Science, 326, 123–125, https://doi.org/10.1126/science.1176985, 2009.
Santoro, A. E., Buchwald, C., McIlvin, M. R., and Casciotti, K. L.: Isotopic signature of N2O produced by marine ammonia-oxidizing archaea, Science, 333, 1282–1285, https://doi.org/10.1126/science.1208239, 2011.
Sasaki, Y., Koba, K., Yamamoto, M., Makabe, A., Ueno, Y., Nakagawa, M., Toyoda, S., Yoshida, N., and Yoh, M.: Biogeochemistry of nitrous oxide in Lake Kizaki, Japan, elucidated by nitrous oxide isotopomer analysis, J. Geophys. Res., 116, G04030, https://doi.org/10.1029/2010JG001589, 2011.
Seidell, A. and Linke, W. F.: Solubilities of inorganic and metal organic compounds, 4th Ed., American Chemical Society, Washington DC, 792 pp., 1965.
Stein, L. Y.: Heterotrophic nitrification and nitrifier denitrification, in: Nitrification, edited by: Ward, B. B., Klotz, M. G., and Arp, D. A., ASM Press, Washington DC, 95–114, 2011.
Stein, L. Y. and Yung, Y. L.: Production, Isotopic composition, and Atmospheric fate of biologically produced nitrous oxide, Annu. Rev. Earth Planet Sci., 31, 329–56, https://doi.org/10.1146/annurev.earth.31.110502.080901, 2003.
Sutka, R. L., Ostrom, N. E., Ostrom, P. H., Gandhi, H., and Breznak, J. A.: Nitrogen isotopomer site preference of N2O produced by Nitrosomonas europaea and Methylococcuscapsulatus Bath, Rapid Commun. Mass Sp., 17, 738–745, https://doi.org/10.1002/rcm.968, 2003.
Sutka, R. L., Ostrom, N. E., Ostrom, P. H., Gandhi, H., and Breznak, J. A.: Erratum Nitrogen isotopomer site preference of N2O produced by Nitrosomonas europaea and Methylococcuscapsulatus Bath, Rapid Commun. Mass Sp., 18, 1411–1412, https://doi.org/10.1002/rcm.1482, 2004.
Sutka, R. L., Ostrom, N. E., Ostrom, P. H., Breznak, J. A., Pitt, A. J., Li, F., and Gandhi, H.: Distinguishing Nitrous Oxide Production from Nitrification and Denitrification on the Basis of Isotopomer Abundances, Appl. Environ. Microbiol., 638–644, https://doi.org/10.1128/AEM.72.1.638-644.2006, 2006.
Sutka, R. L., Adams, G. C., Ostrom, N. E., and Ostrom, P. E.: Isotopologue fractionation during N2O production by fungal denitrification, Rapid Commun. Mass Sp., 22, 3986–3996, https://doi.org/10.1002/rcm.3820, 2008.
Takenaka, T., Tashiro, T., Ozaki, A., Takakubo, H., Yamamoto, Y., Maruyama, T., Nagaosa, K., Kimura, H., and Kato, K.: Planktonic bacterial population dynamics with environmental changes in coastal areas of Suruga Bay, Microbes Environ., 22, 257–267, https://doi.org/10.1264/jsme2.22.257, 2007.
Terry, K. R. and Hooper, A. B.: Hydroxylamine oxidoreductase: a 20-heme, 200,000 molecular weight cytochrome c with unusual denaturation properties which forms a 63,000 molecular weight monomer after heme removal, Biochem., 20, 7026–7032, https://doi.org/10.1021/bi00527a039, 1981.
Toyoda, S. and Yoshida, N.: Determination of nitrogen isotopomers of nitrous oxide on a modified isotope ratio mass spectrometer, Anal. Chem., 71, 4711–4718, https://doi.org/10.1021/ac9904563, 1999.
Toyoda, S., Yoshida, N., Miwa, T., Matsui, Y, Yamagishi, H., and Tsunogai, U.: Production mechanism and global budget of N2O inferred from its isotopomers in the western North Pacific, Geophys. Res. Lett., 29, https://doi.org/10.1029/2001GL014311, 2002.
Toyoda, S., Mutobe, H., Yamagishi, H., Yoshida, N., and Tanji, Y.: Fractionation of N2O isotopomers during production by denitrifier, Soil. Biol. Biochem., 37, 1535–1545, https://doi.org/10.1016/j.soilbio.2005.01.009, 2005.
Toyoda, S., Suzuki, Y., Hattori, S., Yamada, K., Fujii, A., Yoshida, N., Kouno, R., Murayama, K., and Shiomi, H.: Isotopomer analysis of production and consumption mechanisms of N2O and CH4 in an advanced wastewater treatment system, Environ. Sci. Technol., 45, 917–922, https://doi.org/10.1021/es102985u, 2011.
Watmough, N. J., Field, S. J., Hughes, R. J. L., and Richardson, D. J.: The bacterial respiratory nitric oxide reductase, Biochem. Soc. T., 37, 392–399, https://doi.org/10.1042/BST0370392, 2009.
Well, R., Flessa, H., Xing, L., Xiaotang, J., and Romheld, V.: Isotopologue ratios of N2O emitted from microcosms with NH4+ fertilized arable soils under conditions favoring nitrification, Soil Biol. Biochem., 40, 2416–2426, https://doi.org/10.1016/j.soilbio.2008.06.003, 2008.
Wrage, N., Velthof, G. L., van Beusichema, M. L., and Oenema, O.: Role of nitrifier denitrification in the production of nitrous oxide, Soil Biol. Biochem., 33, 1723–1732, 2001.
Yamanaka, T. and Sakano, Y.: Oxidation of hydroxylamine to nitrite catalyzed by hydroxylamine oxidoreductase purified from Nitrosomonas europaea, Curr. Microbiol., 4, 239–244, 1980.
Yamanaka, T. and Shinra, M.: Cytochrome c-552 and cytochrome c-554 derived from Nitrosomonas europaea. Purification, properties, and their function in hydroxylamine oxidation, J. Biochem., 75, 1265–1273, 1974.
Yamanaka, T., Shinra, M., Takahashi, K., and Shibasaka, M.: Highly purified hydroxylamine oxidoreductase from Nitrosomonas europaea: some physicochemical and enzymatic properties, J. Biochem., 86, 1101–1108, 1979.
Yoshida, N.: 15N depleted N2O as a product of nitrification, Nature, 335, 528–529, 1988.
Yoshida, N. and Toyoda, S.: Constraining the atmospheric N2O budget from intramolecular site preference in N2O isotopomers, Nature, 405, 330–334, https://doi.org/10.1038/35012558, 2000.
Zumft, W. G.: Nitric oxide reductases of prokaryotes with emphasis on the respiratory, heme–copper oxidase type, J. Inorg. Biochem., 99, 194–215, https://doi.org/10.1016/j.jinorgbio.2004.09.024, 2005.
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