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Volume 15, issue 1
Biogeosciences, 15, 353–367, 2018
https://doi.org/10.5194/bg-15-353-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 3.0 License.
Biogeosciences, 15, 353–367, 2018
https://doi.org/10.5194/bg-15-353-2018
© Author(s) 2018. This work is distributed under
the Creative Commons Attribution 3.0 License.

Research article 17 Jan 2018

Research article | 17 Jan 2018

Use of flow cytometry and stable isotope analysis to determine phytoplankton uptake of wastewater derived ammonium in a nutrient-rich river

Calla M. Schmidt et al.

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Cited articles

Altabet, M., Pilskaln, C., Thunell, R., Pride, C., Sigman, D., Chavez, F., and Francois, R.: The nitrogen isotope biogeochemistry of sinking particles from the margin of the Eastern North Pacific, Deep-Sea Res. Pt. I, 46, 655–679, https://doi.org/10.1016/S0967-0637(98)00084-3, 1999.
Berg, G. M., Glibert, P. M., Jorgensen, N. O. G., Balode, M., and Purina, I.: Variability in Inorganic and Organic Nitrogen Uptake Associated with Riverine Nutrient Input in the Gulf of Riga, Baltic Sea, Estuaries, 24, 204, https://doi.org/10.2307/1352945, 2001.
Canuel, E. A., Cloern, J. E., Ringelberg, D. B., Guckert, J. B., and Rau, G. H.: Molecular and isotopic tracers used to examine sources of organic matter and its incorporation into the food webs of San Francisco Bay, Limnol. Oceanogr., 40, 67–81, https://doi.org/10.4319/lo.1995.40.1.0067, 1995.
Casciotti, K. L., Sigman, D. M., Hastings, M. G., Böhlke, J. K., and Hilkert, A.: Measurement of the oxygen isotopic composition of nitrate in seawater and freshwater using the denitrifier method, Anal. Chem., 74, 4905–4912, https://doi.org/10.1021/ac020113w, 2002.
Cifuentes, L. A., Fogel, M. L., Pennock, J. R., and Sharp, J. H.: Biogeochemical factors that influence the stable nitrogen isotope ratio of dissolved ammonium in the Delaware Estuary, Geochim. Cosmochim. Ac., 53, 2713–2721, https://doi.org/10.1016/0016-7037(89)90142-7, 1989.
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In this study we measured phytoplankton use of nitrogen from wastewater treatment plant effluent in a river. The project was designed to test a new technique for isolating phytoplankton from river water prior to isotopic analysis in order to use trace nitrogen source use under environmental conditions. This study helps us understand the fate of anthropogenic nitrogen in aquatic ecosystems, which is essential to establishing effective nutrient management plans.
In this study we measured phytoplankton use of nitrogen from wastewater treatment plant effluent...
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